Detailed information of TA system TA118456

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 1273762..1274532
• Replicon: chromosome
• Accession: NZ_CP035770
• Organism: Escherichia coli O145 strain RM8988-C1

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: EW646_RS06140
• Protein ID: WP_001401034.1
• Coordinates: 1274113..1274532 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: EW646_RS06135
• Protein ID: WP_001401035.1
• Coordinates: 1273762..1274028 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EW646_RS06135	1273762..1274028	+	267	WP_001401035.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
EW646_RS06140	1274113..1274532	+	420	WP_001401034.1	HipA domain-containing protein	Toxin
EW646_RS06145	1274710..1274996	+	287	Protein_1197	helix-turn-helix transcriptional regulator	-
EW646_RS06150	1275430..1275993	+	564	WP_000768279.1	type 1 fimbrial major subunit FimA	-
EW646_RS06155	1276354..1277064	+	711	WP_001195165.1	fimbria/pilus periplasmic chaperone	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimA	1268374..1288980	20606

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (35-130)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15706.14 Da
• Isoelectric Point: 5.2713

>T118456 WP_001401034.1 NZ_CP035770:1274113-1274532 [Escherichia coli O145]
MAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTALLRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATL
DLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRWNTTRFSLGFCWCA

Nucleotide

• Length: 420 bp

>T118456 NZ_CP035770:1274113-1274532 [Escherichia coli O145]
ATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAGCAGATATCCCGCTAGGCATGAT
TAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCGCTGCTCAGAATAGGCAATGACTGGT
GCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGGGAAATCAGGCAGCCCAATGCGACGCTC
GATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTGGGTTGAATGTTCCGGACGCAGA
AATCATTAAAGCGGGAAATGTGCGTGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGGAATACTACTAGATTCTCCCTTG
GTTTCTGTTGGTGCGCATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10077.48 Da
• Isoelectric Point: 4.9632

>AT118456 WP_001401035.1 NZ_CP035770:1273762-1274028 [Escherichia coli O145]
MMSFQKIYSPMQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT118456 NZ_CP035770:1273762-1274028 [Escherichia coli O145]
ATGATGAGCTTTCAGAAGATCTATAGCCCAATGCAATTAGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATCGGCATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCACTTGAACTCTCAATGACACTATGCGACACGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAGATCTGGAGTGGTAA