Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 4184738..4185538 Replicon chromosome
Accession NZ_CP035768
Organism Escherichia coli O145 strain RM11626-C1

Toxin (Protein)


Gene name ataT Uniprot ID U9Y257
Locus tag EW649_RS20785 Protein ID WP_000342447.1
Coordinates 4184738..4185265 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID U9Y285
Locus tag EW649_RS20790 Protein ID WP_001277109.1
Coordinates 4185272..4185538 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW649_RS20760 4179812..4180579 - 768 WP_000082096.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
EW649_RS20765 4180576..4181853 - 1278 WP_000803782.1 branched chain amino acid ABC transporter permease LivM -
EW649_RS20770 4181850..4182776 - 927 WP_000003013.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
EW649_RS20775 4182824..4183933 - 1110 WP_001400431.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
EW649_RS20780 4184358..4184741 + 384 WP_000778795.1 aspartate 1-decarboxylase autocleavage activator PanM -
EW649_RS20785 4184738..4185265 - 528 WP_000342447.1 GNAT family N-acetyltransferase Toxin
EW649_RS20790 4185272..4185538 - 267 WP_001277109.1 DUF1778 domain-containing protein Antitoxin
EW649_RS20795 4185688..4186791 - 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
EW649_RS20800 4187062..4187916 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
EW649_RS20805 4188161..4189219 - 1059 WP_001042003.1 permease-like cell division protein FtsX -
EW649_RS20810 4189212..4189880 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19673.63 Da        Isoelectric Point: 7.0284

>T118449 WP_000342447.1 NZ_CP035768:c4185265-4184738 [Escherichia coli O145]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDHSIQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T118449 NZ_CP035768:c4185265-4184738 [Escherichia coli O145]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTCACTCTTGGGCGTCTGGCAATTGATCATTCAATACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCAGTAGGTATTCACGGTCTTTTTGTAG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATAAATCGCTGGGCTTTATCCCGTTGGTCGGGGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATTGAACTGCTTTTTACACAGAGCGATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9938.37 Da        Isoelectric Point: 10.0863

>AT118449 WP_001277109.1 NZ_CP035768:c4185538-4185272 [Escherichia coli O145]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AVKRLQGM

Download         Length: 267 bp

>AT118449 NZ_CP035768:c4185538-4185272 [Escherichia coli O145]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGTAAAACGTCTTCAGGGAATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LUM2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LUN0

References