Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 4184632..4185432 Replicon chromosome
Accession NZ_CP035767
Organism Escherichia coli O145 strain RM12522-C8

Toxin (Protein)


Gene name ataT Uniprot ID U9Y257
Locus tag EW647_RS21235 Protein ID WP_000342447.1
Coordinates 4184632..4185159 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID U9Y285
Locus tag EW647_RS21240 Protein ID WP_001277109.1
Coordinates 4185166..4185432 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW647_RS21210 4179706..4180473 - 768 WP_000082096.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
EW647_RS21215 4180470..4181747 - 1278 WP_000803782.1 branched chain amino acid ABC transporter permease LivM -
EW647_RS21220 4181744..4182670 - 927 WP_000003013.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
EW647_RS21225 4182718..4183827 - 1110 WP_001400431.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
EW647_RS21230 4184252..4184635 + 384 WP_000778795.1 aspartate 1-decarboxylase autocleavage activator PanM -
EW647_RS21235 4184632..4185159 - 528 WP_000342447.1 GNAT family N-acetyltransferase Toxin
EW647_RS21240 4185166..4185432 - 267 WP_001277109.1 DUF1778 domain-containing protein Antitoxin
EW647_RS21245 4185582..4186685 - 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
EW647_RS21250 4186956..4187810 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
EW647_RS21255 4188055..4189113 - 1059 WP_001042003.1 permease-like cell division protein FtsX -
EW647_RS21260 4189106..4189774 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19673.63 Da        Isoelectric Point: 7.0284

>T118425 WP_000342447.1 NZ_CP035767:c4185159-4184632 [Escherichia coli O145]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDHSIQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T118425 NZ_CP035767:c4185159-4184632 [Escherichia coli O145]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTCACTCTTGGGCGTCTGGCAATTGATCATTCAATACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCAGTAGGTATTCACGGTCTTTTTGTAG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATAAATCGCTGGGCTTTATCCCGTTGGTCGGGGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATTGAACTGCTTTTTACACAGAGCGATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9938.37 Da        Isoelectric Point: 10.0863

>AT118425 WP_001277109.1 NZ_CP035767:c4185432-4185166 [Escherichia coli O145]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AVKRLQGM

Download         Length: 267 bp

>AT118425 NZ_CP035767:c4185432-4185166 [Escherichia coli O145]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGTAAAACGTCTTCAGGGAATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LUM2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LUN0

References