Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2016464..2017102 Replicon chromosome
Accession NZ_CP035767
Organism Escherichia coli O145 strain RM12522-C8

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag EW647_RS10440 Protein ID WP_000813794.1
Coordinates 2016926..2017102 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag EW647_RS10435 Protein ID WP_001270286.1
Coordinates 2016464..2016880 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW647_RS10415 2011615..2012556 - 942 WP_001251328.1 ABC transporter permease -
EW647_RS10420 2012557..2013570 - 1014 WP_000220414.1 ABC transporter ATP-binding protein -
EW647_RS10425 2013588..2014733 - 1146 WP_000335342.1 ABC transporter substrate-binding protein -
EW647_RS10430 2014979..2016385 - 1407 WP_000760665.1 PLP-dependent aminotransferase family protein -
EW647_RS10435 2016464..2016880 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
EW647_RS10440 2016926..2017102 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
EW647_RS10445 2017324..2017554 + 231 WP_000494244.1 YncJ family protein -
EW647_RS10450 2017646..2019607 - 1962 WP_000218823.1 23S rRNA 5-hydroxycytidine C2501 synthase -
EW647_RS10455 2019680..2020216 - 537 WP_000429147.1 DNA-binding transcriptional regulator SutR -
EW647_RS10460 2020269..2021480 + 1212 WP_071792349.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T118409 WP_000813794.1 NZ_CP035767:c2017102-2016926 [Escherichia coli O145]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T118409 NZ_CP035767:c2017102-2016926 [Escherichia coli O145]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT118409 WP_001270286.1 NZ_CP035767:c2016880-2016464 [Escherichia coli O145]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT118409 NZ_CP035767:c2016880-2016464 [Escherichia coli O145]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCACGATCATTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAAAT
TACTCGCTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CACTGGTGATGGTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References