Detailed information of TA system TA118408

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 1923424..1924194
• Replicon: chromosome
• Accession: NZ_CP035767
• Organism: Escherichia coli O145 strain RM12522-C8

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: EW647_RS10025
• Protein ID: WP_001401034.1
• Coordinates: 1923775..1924194 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: EW647_RS10020
• Protein ID: WP_001401035.1
• Coordinates: 1923424..1923690 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EW647_RS10020	1923424..1923690	+	267	WP_001401035.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
EW647_RS10025	1923775..1924194	+	420	WP_001401034.1	HipA domain-containing protein	Toxin
EW647_RS10030	1924372..1924658	+	287	Protein_1876	helix-turn-helix transcriptional regulator	-
EW647_RS10035	1925092..1925655	+	564	WP_000768279.1	type 1 fimbrial major subunit FimA	-
EW647_RS10040	1926016..1926726	+	711	WP_001195165.1	fimbria/pilus periplasmic chaperone	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimA	1918037..1938642	20605

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (35-130)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15706.14 Da
• Isoelectric Point: 5.2713

>T118408 WP_001401034.1 NZ_CP035767:1923775-1924194 [Escherichia coli O145]
MAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTALLRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATL
DLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRWNTTRFSLGFCWCA

Nucleotide

• Length: 420 bp

>T118408 NZ_CP035767:1923775-1924194 [Escherichia coli O145]
ATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAGCAGATATCCCGCTAGGCATGAT
TAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCGCTGCTCAGAATAGGCAATGACTGGT
GCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGGGAAATCAGGCAGCCCAATGCGACGCTC
GATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTGGGTTGAATGTTCCGGACGCAGA
AATCATTAAAGCGGGAAATGTGCGTGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGGAATACTACTAGATTCTCCCTTG
GTTTCTGTTGGTGCGCATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10077.48 Da
• Isoelectric Point: 4.9632

>AT118408 WP_001401035.1 NZ_CP035767:1923424-1923690 [Escherichia coli O145]
MMSFQKIYSPMQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT118408 NZ_CP035767:1923424-1923690 [Escherichia coli O145]
ATGATGAGCTTTCAGAAGATCTATAGCCCAATGCAATTAGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATCGGCATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCACTTGAACTCTCAATGACACTATGCGACACGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAGATCTGGAGTGGTAA