Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 483492..484110 Replicon chromosome
Accession NZ_CP035767
Organism Escherichia coli O145 strain RM12522-C8

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag EW647_RS02660 Protein ID WP_001291435.1
Coordinates 483492..483710 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag EW647_RS02665 Protein ID WP_000344800.1
Coordinates 483736..484110 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW647_RS02625 478781..479353 + 573 WP_000779831.1 YbaY family lipoprotein -
EW647_RS02630 479384..479695 - 312 WP_000409911.1 MGMT family protein -
EW647_RS02640 480074..480427 + 354 WP_000878140.1 DUF1428 domain-containing protein -
EW647_RS02645 480469..482019 - 1551 WP_024221674.1 cyclic-guanylate-specific phosphodiesterase PdeB -
EW647_RS02650 482183..482653 - 471 WP_000136192.1 YlaC family protein -
EW647_RS02655 482769..483320 - 552 WP_000102564.1 maltose O-acetyltransferase -
EW647_RS02660 483492..483710 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
EW647_RS02665 483736..484110 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
EW647_RS02670 484656..487805 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
EW647_RS02675 487828..489021 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T118406 WP_001291435.1 NZ_CP035767:c483710-483492 [Escherichia coli O145]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T118406 NZ_CP035767:c483710-483492 [Escherichia coli O145]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT118406 WP_000344800.1 NZ_CP035767:c484110-483736 [Escherichia coli O145]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT118406 NZ_CP035767:c484110-483736 [Escherichia coli O145]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References