Detailed information of TA system TA118401

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 31773..32308
• Replicon: plasmid pE110019_65
• Accession: NZ_CP035753
• Organism: Escherichia coli E110019

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: ECE110019_RS26410
• Protein ID: WP_001270421.1
• Coordinates: 31773..32060 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: L4J0V6
• Locus tag: ECE110019_RS26415
• Protein ID: WP_001132895.1
• Coordinates: 32057..32308 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECE110019_RS26380	26967..28403	+	1437	WP_000708193.1	alpha-hemolysin T1SS ABC transporter subunit HlyD	-
ECE110019_RS26385	28476..29090	-	615	Protein_29	IS3-like element IS600 family transposase	-
ECE110019_RS26390	29145..30358	+	1214	WP_164474067.1	IS3 family transposase	-
ECE110019_RS26395	30401..31081	-	681	Protein_31	IS30 family transposase	-
ECE110019_RS26400	31170..31451	-	282	Protein_32	IS3 family transposase	-
ECE110019_RS26405	31490..31768	-	279	Protein_33	DDE-type integrase/transposase/recombinase	-
ECE110019_RS26410	31773..32060	-	288	WP_001270421.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ECE110019_RS26415	32057..32308	-	252	WP_001132895.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
ECE110019_RS26420	32482..32694	-	213	WP_000402946.1	hypothetical protein	-
ECE110019_RS26425	33068..34273	+	1206	WP_000817645.1	AAA family ATPase	-
ECE110019_RS26430	34270..35232	+	963	WP_000756323.1	ParB/RepB/Spo0J family partition protein	-
ECE110019_RS26435	35373..36555	-	1183	Protein_39	translesion error-prone DNA polymerase V subunit UmuC	-
ECE110019_RS26440	36496..36768	+	273	WP_000952680.1	antirestriction protein	-
ECE110019_RS26445	36815..37237	+	423	WP_000271766.1	DUF1380 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	espT / pet / hlyC / hlyA / hlyB / hlyD	1..65378	65378

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11244.27 Da
• Isoelectric Point: 10.4703

>T118401 WP_001270421.1 NZ_CP035753:c32060-31773 [Escherichia coli E110019]
MRYQVKFREDALKEWQKLDKTIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Nucleotide

• Length: 288 bp

>T118401 NZ_CP035753:c32060-31773 [Escherichia coli E110019]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAACTGGACAAGACTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGTGACAATCCGCATATTCCTTCCGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCTTGGTCTATCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCTGTGGGTAAA
CGTGAGCGTAGTGACGTTTATAATCTTGCCAGTGAAAGAATGAGATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9178.47 Da
• Isoelectric Point: 4.2108

>AT118401 WP_001132895.1 NZ_CP035753:c32308-32057 [Escherichia coli E110019]
MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Nucleotide

• Length: 252 bp

>AT118401 NZ_CP035753:c32308-32057 [Escherichia coli E110019]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAATCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGGTCGCTATCCTGAACCGTAATCAGCCTGCTTTCTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TCGATGCCCTGGACGATCAGGAGCTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTAGACCTGGAT
AAATACCTATGA

Structures

Antitoxin

• AlphaFold DB: L4J0V6