Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 44780..45327 Replicon plasmid pE110019_66
Accession NZ_CP035752
Organism Escherichia coli E110019

Toxin (Protein)


Gene name parE Uniprot ID E9LLH3
Locus tag ECE110019_RS26085 Protein ID WP_000969996.1
Coordinates 45046..45327 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID F4V9S6
Locus tag ECE110019_RS26080 Protein ID WP_000079931.1
Coordinates 44780..45049 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECE110019_RS26040 40412..40624 + 213 WP_001299730.1 ANR family transcriptional regulator -
ECE110019_RS26045 40871..41332 + 462 WP_000978818.1 thermonuclease family protein -
ECE110019_RS26050 41378..41587 + 210 WP_001299729.1 hemolysin expression modulator Hha -
ECE110019_RS26055 41626..42216 + 591 WP_000766816.1 DUF2726 domain-containing protein -
ECE110019_RS26060 42456..42716 + 261 WP_000083819.1 replication regulatory protein RepA -
ECE110019_RS26065 42941..43015 + 75 WP_001365705.1 RepA leader peptide Tap -
ECE110019_RS26070 43008..43865 + 858 WP_000131009.1 incFII family plasmid replication initiator RepA -
ECE110019_RS26080 44780..45049 + 270 WP_000079931.1 hypothetical protein Antitoxin
ECE110019_RS26085 45046..45327 + 282 WP_000969996.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ECE110019_RS26090 45419..45942 - 524 Protein_51 IS1 family transposase -
ECE110019_RS26095 46469..46919 + 451 Protein_52 hypothetical protein -
ECE110019_RS26100 47072..47776 + 705 WP_001067859.1 IS6 family transposase -
ECE110019_RS26105 47914..48978 - 1065 WP_000946279.1 fimbrial protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - mrkJ / mrkI 1..66171 66171
- flank IS/Tn - - 47072..47776 704


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10893.57 Da        Isoelectric Point: 8.4615

>T118400 WP_000969996.1 NZ_CP035752:45046-45327 [Escherichia coli E110019]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T118400 NZ_CP035752:45046-45327 [Escherichia coli E110019]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGATAGCCAGTGGGGAAAAGTG
TATATCCTGCGCGTCCTGCATACAGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10194.55 Da        Isoelectric Point: 8.8735

>AT118400 WP_000079931.1 NZ_CP035752:44780-45049 [Escherichia coli E110019]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT118400 NZ_CP035752:44780-45049 [Escherichia coli E110019]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATTCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACTTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAACGCGGCGATGTGCTTCCCCATGACATGGTCGAATCCTCTGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTCGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E9LLH3

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DLR5

References