Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 5174161..5174658 Replicon chromosome
Accession NZ_CP035751
Organism Escherichia coli E110019

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag ECE110019_RS25425 Protein ID WP_001173343.1
Coordinates 5174485..5174658 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag ECE110019_RS25420 Protein ID WP_000398619.1
Coordinates 5174161..5174457 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECE110019_RS25400 5170892..5171611 - 720 WP_000611278.1 two-component system response regulator DcuR -
ECE110019_RS25405 5171608..5173239 - 1632 WP_001216487.1 sensor histidine kinase -
ECE110019_RS25410 5173420..5173650 + 231 WP_000371700.1 (4Fe-4S)-binding protein -
ECE110019_RS25415 5173662..5173934 + 273 WP_000405647.1 N-acetyltransferase -
ECE110019_RS25420 5174161..5174457 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
ECE110019_RS25425 5174485..5174658 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
ECE110019_RS25430 5174777..5176294 - 1518 WP_001295091.1 lysine--tRNA ligase -
ECE110019_RS25435 5176531..5177988 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T118395 WP_001173343.1 NZ_CP035751:5174485-5174658 [Escherichia coli E110019]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T118395 NZ_CP035751:5174485-5174658 [Escherichia coli E110019]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT118395 WP_000398619.1 NZ_CP035751:5174161-5174457 [Escherichia coli E110019]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT118395 NZ_CP035751:5174161-5174457 [Escherichia coli E110019]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References