Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3794102..3794756 Replicon chromosome
Accession NZ_CP035751
Organism Escherichia coli E110019

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag ECE110019_RS18830 Protein ID WP_000244777.1
Coordinates 3794102..3794509 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag ECE110019_RS18835 Protein ID WP_000354046.1
Coordinates 3794490..3794756 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECE110019_RS18810 3790059..3791792 - 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -
ECE110019_RS18815 3791798..3792508 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
ECE110019_RS18820 3792533..3793429 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
ECE110019_RS18825 3793541..3794062 + 522 WP_001055874.1 flavodoxin FldB -
ECE110019_RS18830 3794102..3794509 - 408 WP_000244777.1 toxin CptA Toxin
ECE110019_RS18835 3794490..3794756 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
ECE110019_RS18840 3794999..3795979 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
ECE110019_RS18845 3796175..3796834 - 660 WP_000250274.1 hemolysin III family protein -
ECE110019_RS18850 3796998..3797309 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
ECE110019_RS18855 3797354..3798787 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
ECE110019_RS18860 3798844..3799587 - 744 WP_024184477.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T118392 WP_000244777.1 NZ_CP035751:c3794509-3794102 [Escherichia coli E110019]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T118392 NZ_CP035751:c3794509-3794102 [Escherichia coli E110019]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT118392 WP_000354046.1 NZ_CP035751:c3794756-3794490 [Escherichia coli E110019]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT118392 NZ_CP035751:c3794756-3794490 [Escherichia coli E110019]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References