Detailed information of TA system TA118390

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3307010..3307635
• Replicon: chromosome
• Accession: NZ_CP035751
• Organism: Escherichia coli E110019

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: ECE110019_RS16450
• Protein ID: WP_000911330.1
• Coordinates: 3307010..3307408 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: ECE110019_RS16455
• Protein ID: WP_000450524.1
• Coordinates: 3307408..3307635 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECE110019_RS16430	3302888..3303088	+	201	WP_000383836.1	YpfN family protein	-
ECE110019_RS16435	3303198..3303896	-	699	WP_000679812.1	esterase	-
ECE110019_RS16440	3303970..3305985	-	2016	WP_000829394.1	tRNA cytosine(34) acetyltransferase TmcA	-
ECE110019_RS16445	3306000..3306863	-	864	WP_001267498.1	neutral zinc metallopeptidase	-
ECE110019_RS16450	3307010..3307408	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
ECE110019_RS16455	3307408..3307635	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
ECE110019_RS16460	3307789..3308502	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
ECE110019_RS16465	3308715..3309749	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
ECE110019_RS16470	3309766..3310644	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
ECE110019_RS16475	3310790..3311362	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
ECE110019_RS16480	3311362..3311832	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T118390 WP_000911330.1 NZ_CP035751:c3307408-3307010 [Escherichia coli E110019]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T118390 NZ_CP035751:c3307408-3307010 [Escherichia coli E110019]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTAATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT118390 WP_000450524.1 NZ_CP035751:c3307635-3307408 [Escherichia coli E110019]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT118390 NZ_CP035751:c3307635-3307408 [Escherichia coli E110019]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33