Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1975278..1975916 Replicon chromosome
Accession NZ_CP035751
Organism Escherichia coli E110019

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag ECE110019_RS09810 Protein ID WP_000813794.1
Coordinates 1975278..1975454 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag ECE110019_RS09815 Protein ID WP_001270286.1
Coordinates 1975500..1975916 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECE110019_RS09790 1970897..1972111 - 1215 WP_071821428.1 BenE family transporter YdcO -
ECE110019_RS09795 1972164..1972700 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
ECE110019_RS09800 1972773..1974734 + 1962 WP_001298803.1 23S rRNA 5-hydroxycytidine C2501 synthase -
ECE110019_RS09805 1974826..1975056 - 231 WP_000494244.1 YncJ family protein -
ECE110019_RS09810 1975278..1975454 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
ECE110019_RS09815 1975500..1975916 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
ECE110019_RS09820 1975995..1977401 + 1407 WP_000760638.1 PLP-dependent aminotransferase family protein -
ECE110019_RS09825 1977646..1978791 + 1146 WP_000047456.1 ABC transporter substrate-binding protein -
ECE110019_RS09830 1978809..1979822 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
ECE110019_RS09835 1979823..1980646 + 824 Protein_1925 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1969709..1970857 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T118377 WP_000813794.1 NZ_CP035751:1975278-1975454 [Escherichia coli E110019]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T118377 NZ_CP035751:1975278-1975454 [Escherichia coli E110019]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT118377 WP_001270286.1 NZ_CP035751:1975500-1975916 [Escherichia coli E110019]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT118377 NZ_CP035751:1975500-1975916 [Escherichia coli E110019]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References