Detailed information of TA system TA118374

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 637969..638806
• Replicon: chromosome
• Accession: NZ_CP035751
• Organism: Escherichia coli E110019

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: ECE110019_RS02990
• Protein ID: WP_000227784.1
• Coordinates: 638264..638806 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: ECE110019_RS02985
• Protein ID: WP_001297137.1
• Coordinates: 637969..638280 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECE110019_RS02960	632989..633936	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
ECE110019_RS02965	633958..635949	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
ECE110019_RS02970	635939..636553	+	615	WP_000179821.1	cytochrome o ubiquinol oxidase subunit III	-
ECE110019_RS02975	636553..636882	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
ECE110019_RS02980	636894..637784	+	891	WP_000971341.1	heme o synthase	-
ECE110019_RS02985	637969..638280	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
ECE110019_RS02990	638264..638806	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
ECE110019_RS02995	638862..639797	-	936	WP_001299550.1	sel1 repeat family protein	-
ECE110019_RS03000	640205..641569	+	1365	WP_001000978.1	MFS transporter	-
ECE110019_RS03005	641697..642188	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
ECE110019_RS03010	642356..643267	+	912	WP_000705865.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T118374 WP_000227784.1 NZ_CP035751:638264-638806 [Escherichia coli E110019]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T118374 NZ_CP035751:638264-638806 [Escherichia coli E110019]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT118374 WP_001297137.1 NZ_CP035751:637969-638280 [Escherichia coli E110019]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT118374 NZ_CP035751:637969-638280 [Escherichia coli E110019]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3