Detailed information of TA system TA118363

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4195910..4196486
• Replicon: chromosome
• Accession: NZ_CP035738
• Organism: Enterobacter cloacae strain CZ-1

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: EWI30_RS20495
• Protein ID: WP_048377779.1
• Coordinates: 4196199..4196486 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: EWI30_RS20490
• Protein ID: WP_128348631.1
• Coordinates: 4195910..4196212 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EWI30_RS20465	4191651..4191854	+	204	WP_003856610.1	YbdD/YjiX family protein	-
EWI30_RS20470	4191865..4192818	+	954	WP_114979208.1	GTPase	-
EWI30_RS20475	4192873..4194231	-	1359	WP_129996760.1	esterase-like activity of phytase family protein	-
EWI30_RS20480	4194449..4195192	+	744	WP_129996761.1	AraC family transcriptional regulator	-
EWI30_RS20485	4195241..4195879	+	639	WP_129996762.1	LysE family translocator	-
EWI30_RS20490	4195910..4196212	-	303	WP_128348631.1	BrnA antitoxin family protein	Antitoxin
EWI30_RS20495	4196199..4196486	-	288	WP_048377779.1	BrnT family toxin	Toxin
EWI30_RS20500	4196656..4197924	+	1269	WP_129996763.1	DUF445 domain-containing protein	-
EWI30_RS20505	4197914..4198789	-	876	WP_129996764.1	LysR family transcriptional regulator	-
EWI30_RS20510	4198875..4199183	+	309	WP_129996765.1	carboxymuconolactone decarboxylase family protein	-
EWI30_RS20515	4199183..4199563	+	381	WP_129996766.1	tautomerase family protein	-
EWI30_RS20520	4199554..4200618	-	1065	WP_129996767.1	DUF2955 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11265.76 Da
• Isoelectric Point: 7.4007

>T118363 WP_048377779.1 NZ_CP035738:c4196486-4196199 [Enterobacter cloacae]
MPMEYEWDSNKAKSNLQKHGIRFEDAVMVFDDPYHLSVQDRYENGEFRWQTIGLVQGLLVILVAHTVRFESGGEIIRIIS
ARKADRKERSRYEHR

Nucleotide

• Length: 288 bp

>T118363 NZ_CP035738:c4196486-4196199 [Enterobacter cloacae]
ATGCCAATGGAGTATGAGTGGGATAGCAATAAGGCGAAAAGTAACCTGCAAAAACATGGCATACGCTTTGAGGATGCGGT
TATGGTTTTCGACGATCCTTATCATTTGTCTGTTCAGGATCGCTATGAGAACGGTGAATTTCGCTGGCAAACCATCGGCC
TTGTGCAAGGCCTTCTTGTTATTCTGGTCGCACATACGGTGCGTTTTGAAAGCGGAGGAGAAATCATCCGTATAATTAGC
GCACGAAAGGCGGACCGCAAAGAGAGGAGCCGTTATGAGCATCGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11469.15 Da
• Isoelectric Point: 10.0592

>AT118363 WP_128348631.1 NZ_CP035738:c4196212-4195910 [Enterobacter cloacae]
MSIVKHKRGSAPEVSPRREAELKALAEKPEAEIDYSDIPAMDDEQWSGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT118363 NZ_CP035738:c4196212-4195910 [Enterobacter cloacae]
ATGAGCATCGTTAAACATAAACGAGGTAGTGCGCCTGAGGTAAGCCCTCGTCGTGAGGCTGAGCTCAAAGCATTAGCTGA
AAAACCAGAAGCAGAGATTGATTACAGCGATATCCCTGCTATGGACGATGAACAGTGGTCAGGGGCGGTGCGAGGGAAAT
TTTATCGACCACTCAAAACTCAGGCATCTGTACGCATTGACGCCGATGTGATGGAGTGGCTTAAACGGCCTGGGAAAGGT
TACCAGACGCGGCTCAATGCCATTTTGCGTGAAGCAATGCTGCGCGATCAAAACAAAAAATAA