Detailed information of TA system TA118362

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 3847950..3848665
• Replicon: chromosome
• Accession: NZ_CP035738
• Organism: Enterobacter cloacae strain CZ-1

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: EWI30_RS18875
• Protein ID: WP_114977863.1
• Coordinates: 3847950..3848318 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: EWI30_RS18880
• Protein ID: WP_129996617.1
• Coordinates: 3848339..3848665 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EWI30_RS18850	3843293..3844339	+	1047	WP_129996613.1	L-asparaginase 2	-
EWI30_RS18855	3844445..3844849	-	405	WP_129996614.1	hypothetical protein	-
EWI30_RS18860	3845194..3845865	+	672	WP_129996615.1	hypothetical protein	-
EWI30_RS18865	3846148..3846468	+	321	Protein_3625	hypothetical protein	-
EWI30_RS18870	3846608..3846988	+	381	WP_129996616.1	hypothetical protein	-
EWI30_RS18875	3847950..3848318	-	369	WP_114977863.1	TA system toxin CbtA family protein	Toxin
EWI30_RS18880	3848339..3848665	-	327	WP_129996617.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EWI30_RS18885	3848678..3849148	-	471	WP_129996618.1	DNA repair protein RadC	-
EWI30_RS18890	3849219..3850037	-	819	WP_114977860.1	DUF945 domain-containing protein	-
EWI30_RS18895	3850188..3850985	-	798	WP_129996619.1	hypothetical protein	-
EWI30_RS18900	3851429..3852313	-	885	WP_114977858.1	50S ribosome-binding GTPase	-
EWI30_RS18905	3852522..3853376	-	855	WP_129996620.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3842200..3865633	23433

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (7-107)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13678.87 Da
• Isoelectric Point: 7.2907

>T118362 WP_114977863.1 NZ_CP035738:c3848318-3847950 [Enterobacter cloacae]
MNTLPVIIPQAAKQGLPPVTVWQMLLTRLLEQHYGLTLHDTPFSDEAVIQEHIDAGITLANAVNFLVEKYELVRIDRCGF
SSQVQAPYLTATDILHARKACGLMSRCSYREVSNIVLSRSRL

Nucleotide

• Length: 369 bp

>T118362 NZ_CP035738:c3848318-3847950 [Enterobacter cloacae]
ATGAATACTCTACCTGTAATCATCCCACAGGCGGCGAAGCAAGGTCTGCCGCCCGTAACTGTCTGGCAAATGTTACTTAC
TCGCCTGCTTGAACAGCACTATGGTCTGACGCTACACGACACACCTTTTAGTGATGAAGCCGTCATACAGGAACATATCG
ATGCCGGTATCACTCTGGCTAATGCCGTTAACTTTCTGGTGGAGAAGTATGAGTTAGTCCGTATCGATCGCTGTGGATTT
TCCTCACAGGTACAAGCACCATATTTGACTGCGACAGACATTCTTCATGCCAGGAAAGCGTGCGGGTTGATGAGCCGGTG
TTCTTACAGGGAGGTAAGTAATATTGTTCTTAGCCGATCACGGCTGTGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12142.82 Da
• Isoelectric Point: 6.2032

>AT118362 WP_129996617.1 NZ_CP035738:c3848665-3848339 [Enterobacter cloacae]
MSFIPAHEWGLKSDIVPRFGARLVQEGNRLHYLADRAGLMGMSSDTECLKLDEAFPHFISQMESMLTTGELNSRHAHCVT
LYHNGFTCEADTLGSCGYVYIVVYPTQR

Nucleotide

• Length: 327 bp

>AT118362 NZ_CP035738:c3848665-3848339 [Enterobacter cloacae]
TTGTCATTTATACCAGCCCATGAATGGGGGCTAAAAAGCGATATTGTTCCACGGTTTGGTGCCAGATTAGTTCAGGAAGG
CAACCGGCTACATTACCTGGCAGACAGGGCTGGGTTGATGGGAATGTCCAGCGATACCGAATGTCTTAAACTGGATGAAG
CATTTCCGCACTTTATCAGTCAGATGGAATCAATGTTGACCACTGGCGAGCTGAATTCCCGCCATGCCCATTGCGTCACC
CTGTACCACAACGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTGCGGCTACGTTTACATCGTCGTCTATCCCACTCA
ACGCTAA