118361

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3719002..3719622 Replicon chromosome
Accession NZ_CP035738
Organism Enterobacter cloacae strain CZ-1

Toxin (Protein)


Gene name Hha Uniprot ID A0A1H8SI38
Locus tag EWI30_RS18240 Protein ID WP_008499287.1
Coordinates 3719404..3719622 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag EWI30_RS18235 Protein ID WP_008499288.1
Coordinates 3719002..3719376 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EWI30_RS18225 3714127..3715320 + 1194 WP_114977962.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
EWI30_RS18230 3715343..3718489 + 3147 WP_129996550.1 multidrug efflux RND transporter permease subunit AcrB -
EWI30_RS18235 3719002..3719376 + 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
EWI30_RS18240 3719404..3719622 + 219 WP_008499287.1 hemolysin expression modulator Hha Toxin
EWI30_RS18245 3719832..3720383 + 552 WP_129996551.1 maltose O-acetyltransferase -
EWI30_RS18250 3720502..3720969 + 468 WP_129996552.1 hypothetical protein -
EWI30_RS18255 3720941..3722401 - 1461 WP_129996553.1 PLP-dependent aminotransferase family protein -
EWI30_RS18260 3722503..3723213 + 711 WP_129996554.1 GNAT family N-acetyltransferase -
EWI30_RS18265 3723210..3723350 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
EWI30_RS18270 3723353..3723613 - 261 WP_010428154.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8612.00 Da        Isoelectric Point: 8.9008

>T118361 WP_008499287.1 NZ_CP035738:3719404-3719622 [Enterobacter cloacae]
MSDKPLTKIDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Download         Length: 219 bp

>T118361 NZ_CP035738:3719404-3719622 [Enterobacter cloacae]
ATGTCTGATAAACCACTCACAAAGATCGATTATTTGATGCGTTTACGACGTTGTCAGTCAATTGACACGCTCGAACGTGT
CATTGAAAAGAATAAATACGAGCTTTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCTGACCATCGTCTTGCTGAAC
TGACGATGAATAAGTTGTATGACAAAATTCCTACTTCTGTATGGAAATTTGTCCGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT118361 WP_008499288.1 NZ_CP035738:3719002-3719376 [Enterobacter cloacae]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT118361 NZ_CP035738:3719002-3719376 [Enterobacter cloacae]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGTAACCATGGCTGGGTGAACGACCCAACGTCTGCCATCAATTTACAGTTGAATGAGCTGATAGAGC
ATATCGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTCATTGAGCAAATTGATGAATACCTG
GATGACACCTTTATGTTGTTCAGCAGTTACGGCATTAACGCCCAGGATTTGCAAAAATGGCGTAAATCGGGAAACCGGCT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCGGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1H8SI38

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References