Detailed information of TA system TA118359

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 3270167..3271080
• Replicon: chromosome
• Accession: NZ_CP035738
• Organism: Enterobacter cloacae strain CZ-1

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: EWI30_RS16055
• Protein ID: WP_129996318.1
• Coordinates: 3270607..3271080 (+)
• Length: 158 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: EWI30_RS16050
• Protein ID: WP_129996317.1
• Coordinates: 3270167..3270610 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EWI30_RS16035	3265750..3267471	+	1722	WP_129996316.1	cysteine/glutathione ABC transporter ATP-binding protein/permease CydC	-
EWI30_RS16040	3267518..3268222	+	705	WP_114978533.1	leucyl/phenylalanyl-tRNA--protein transferase	-
EWI30_RS16045	3268507..3268725	+	219	WP_002211347.1	translation initiation factor IF-1	-
EWI30_RS16050	3270167..3270610	+	444	WP_129996317.1	DUF2384 domain-containing protein	Antitoxin
EWI30_RS16055	3270607..3271080	+	474	WP_129996318.1	RES domain-containing protein	Toxin
EWI30_RS16060	3271103..3271657	-	555	WP_129996319.1	type I-F CRISPR-associated endoribonuclease Cas6/Csy4	-
EWI30_RS16065	3271667..3272665	-	999	WP_129996320.1	type I-F CRISPR-associated protein Csy3	-
EWI30_RS16070	3272679..3273614	-	936	WP_129996321.1	type I-F CRISPR-associated protein Csy2	-
EWI30_RS16075	3273611..3274921	-	1311	WP_129996322.1	type I-F CRISPR-associated protein Csy1	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (34-90)

Sequences

Toxin

Protein

• Length: 158 a.a.
• Molecular weight: 17645.07 Da
• Isoelectric Point: 5.5361

>T118359 WP_129996318.1 NZ_CP035738:3270607-3271080 [Enterobacter cloacae]
MIFYRLVTGRYVSEAWSGSGANQYGGRWNHKGHPAVYVSTSISLASLEILVHVKKDTVLNQYQLFSINIPDDQIDYLDKT
WLPTDWQENPAPVSTMDLGTGWLQANSALALILPSCIIPFENNAIINPLHPEFQKTLNSVQQLPFIFDTRLAEKTAP

Nucleotide

• Length: 474 bp

>T118359 NZ_CP035738:3270607-3271080 [Enterobacter cloacae]
ATGATTTTTTATCGGCTGGTGACGGGGCGTTATGTCAGCGAAGCGTGGAGTGGAAGTGGCGCAAACCAGTATGGTGGTCG
CTGGAATCATAAAGGACACCCTGCCGTGTATGTGTCCACCTCCATTTCACTGGCATCCCTGGAAATCCTGGTGCACGTTA
AAAAAGATACCGTGCTGAATCAATATCAGCTTTTCAGCATTAATATCCCGGATGACCAGATTGATTACCTGGATAAAACG
TGGCTACCCACAGACTGGCAGGAAAACCCAGCACCGGTGTCAACGATGGATCTGGGAACCGGTTGGTTACAGGCTAACAG
TGCCCTCGCACTCATTTTACCTTCCTGCATCATTCCTTTTGAAAACAATGCCATAATTAACCCCTTACACCCTGAATTCC
AAAAGACACTCAATTCTGTACAGCAGCTTCCTTTTATTTTTGATACCCGTCTTGCAGAGAAAACCGCACCCTAA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16411.70 Da
• Isoelectric Point: 7.2051

>AT118359 WP_129996317.1 NZ_CP035738:3270167-3270610 [Enterobacter cloacae]
MRTWIPAQKPADNALWRYAGLPANRGMRLVEYLNQGLPVSVIDNIHEWTAMSKADILRVTGINERNVARRKSTGRTLTPD
ESERVARFVRVLDAAVDYFGSKEEAWNWLQTPVRGLGNVAPVDLIATETGALEVTDLIGRLEHGVFA

Nucleotide

• Length: 444 bp

>AT118359 NZ_CP035738:3270167-3270610 [Enterobacter cloacae]
ATGAGAACATGGATCCCCGCACAGAAACCTGCTGATAACGCACTTTGGCGCTACGCCGGTTTACCTGCAAACAGAGGTAT
GCGGCTGGTTGAATATCTCAATCAGGGGTTACCCGTCAGCGTTATCGACAATATCCATGAATGGACGGCAATGTCGAAAG
CGGACATTCTCCGTGTCACAGGAATTAACGAGCGTAACGTTGCCCGCCGCAAAAGCACGGGTCGAACGCTGACGCCCGAT
GAGAGCGAGCGTGTGGCACGTTTCGTTCGGGTGCTGGATGCAGCTGTTGATTATTTTGGTAGTAAAGAAGAGGCATGGAA
CTGGCTGCAAACGCCAGTGCGTGGGCTTGGGAATGTTGCGCCTGTCGACCTGATTGCCACCGAAACCGGTGCACTGGAAG
TCACCGATTTAATTGGTCGCCTGGAACACGGAGTGTTTGCATGA