Detailed information of TA system TA118357

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 2994051..2994848
• Replicon: chromosome
• Accession: NZ_CP035738
• Organism: Enterobacter cloacae strain CZ-1

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: EWI30_RS14690
• Protein ID: WP_129996140.1
• Coordinates: 2994051..2994572 (-)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A2T4XZW7
• Locus tag: EWI30_RS14695
• Protein ID: WP_028019121.1
• Coordinates: 2994579..2994848 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EWI30_RS14660	2989628..2990317	+	690	WP_129996136.1	pyrimidine utilization protein B	-
EWI30_RS14665	2990329..2990715	+	387	WP_028019115.1	pyrimidine utilization protein C	-
EWI30_RS14670	2990723..2991523	+	801	WP_129996137.1	pyrimidine utilization protein D	-
EWI30_RS14675	2991533..2992123	+	591	WP_129996138.1	malonic semialdehyde reductase	-
EWI30_RS14680	2992133..2992627	+	495	WP_129997110.1	pyrimidine utilization flavin reductase protein F	-
EWI30_RS14685	2992649..2993977	+	1329	WP_129996139.1	pyrimidine utilization transport protein G	-
EWI30_RS14690	2994051..2994572	-	522	WP_129996140.1	GNAT family N-acetyltransferase	Toxin
EWI30_RS14695	2994579..2994848	-	270	WP_028019121.1	DUF1778 domain-containing protein	Antitoxin
EWI30_RS14700	2994921..2995826	-	906	WP_114978273.1	DMT family transporter	-
EWI30_RS14705	2995943..2996113	-	171	WP_001273664.1	general stress protein	-
EWI30_RS14710	2996505..2997101	+	597	WP_114978274.1	NAD(P)H:quinone oxidoreductase	-
EWI30_RS14715	2997120..2997347	+	228	WP_028019124.1	YccJ family protein	-
EWI30_RS14720	2997380..2998621	-	1242	WP_129996141.1	bifunctional glucose-1-phosphatase/inositol phosphatase	-
EWI30_RS14725	2998773..2999216	+	444	WP_114978276.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (2-88)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19763.50 Da
• Isoelectric Point: 7.1161

>T118357 WP_129996140.1 NZ_CP035738:c2994572-2994051 [Enterobacter cloacae]
VENLTIEMFSEGTEYAFEDFDCGEPSLNAFLREHLVRQHNGRILRGYLLKDRDHPHVLGYYTLSGSCFEKAMLPSKTQQR
RIPYINVPSVTLGRLAVHKTLQGNEWGTTLVTHAMRVVYLASQAVGVHGLFVDALNEQAKRFYLKQGFIPLQGDNSNSLF
FPTKSIERLFEQE

Nucleotide

• Length: 522 bp

>T118357 NZ_CP035738:c2994572-2994051 [Enterobacter cloacae]
GTGGAAAATCTCACGATCGAAATGTTTTCAGAGGGAACCGAATACGCTTTCGAGGATTTCGATTGTGGTGAGCCCTCGCT
AAATGCCTTCCTGAGAGAGCATCTGGTGCGCCAGCACAATGGGCGCATTTTGCGCGGCTATCTGCTAAAAGATCGTGACC
ATCCACACGTGCTGGGCTACTACACGTTGTCTGGAAGTTGCTTTGAAAAAGCGATGCTCCCTTCTAAGACGCAGCAGCGC
AGGATCCCCTATATCAACGTACCCAGTGTGACTTTGGGACGTTTAGCTGTTCATAAAACACTACAGGGAAATGAATGGGG
GACAACACTCGTCACGCATGCTATGCGGGTTGTTTACCTGGCTTCACAGGCTGTGGGTGTGCATGGCCTTTTTGTTGATG
CGCTTAATGAGCAGGCAAAAAGGTTCTATCTGAAGCAAGGTTTTATTCCGCTGCAAGGGGATAACAGCAATTCTCTGTTC
TTCCCTACAAAATCTATCGAACGACTGTTTGAGCAAGAGTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10220.68 Da
• Isoelectric Point: 5.8770

>AT118357 WP_028019121.1 NZ_CP035738:c2994848-2994579 [Enterobacter cloacae]
MPALKKQRIDLRLTDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIDQHRRLVLNEASWNAVMDAIDNPPEPNERLKR
AAKRLKNME

Nucleotide

• Length: 270 bp

>AT118357 NZ_CP035738:c2994848-2994579 [Enterobacter cloacae]
ATGCCAGCACTTAAAAAACAGCGTATCGATCTTCGCTTAACGGACGAAGACAAAACAATGATTGAAGAAGCGGCGGCGAT
GACGAATCAAACGATCACTCAGTTTATGGTGAACAGTGCTTCGGAACGTGCTGCAGAAGTCATCGATCAGCATCGACGTC
TCGTATTAAACGAAGCATCGTGGAATGCCGTAATGGATGCCATCGACAATCCACCCGAGCCGAATGAACGCCTGAAGCGA
GCCGCGAAGCGTCTTAAAAACATGGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2T4XZW7