Detailed information of TA system TA118348

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1740030..1740620
• Replicon: chromosome
• Accession: NZ_CP035738
• Organism: Enterobacter cloacae strain CZ-1

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: EWI30_RS08430
• Protein ID: WP_129995437.1
• Coordinates: 1740288..1740620 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: EWI30_RS08425
• Protein ID: WP_129995436.1
• Coordinates: 1740030..1740287 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EWI30_RS08395	1735484..1736434	+	951	WP_129995431.1	HTH-type transcriptional regulator Cbl	-
EWI30_RS08400	1736479..1737087	-	609	WP_129995432.1	glutathione S-transferase family protein	-
EWI30_RS08405	1737258..1738151	+	894	WP_129995433.1	LysR family transcriptional regulator	-
EWI30_RS08410	1738280..1739209	-	930	WP_129995434.1	LysR family transcriptional regulator	-
EWI30_RS08415	1739351..1739740	+	390	WP_129995435.1	RidA family protein	-
EWI30_RS08425	1740030..1740287	+	258	WP_129995436.1	antitoxin	Antitoxin
EWI30_RS08430	1740288..1740620	+	333	WP_129995437.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
EWI30_RS08440	1740920..1743109	-	2190	WP_114979740.1	TonB-dependent siderophore receptor	-
EWI30_RS08445	1743136..1744461	-	1326	WP_129995438.1	SidA/IucD/PvdA family monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-109)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11856.85 Da
• Isoelectric Point: 10.5834

>T118348 WP_129995437.1 NZ_CP035738:1740288-1740620 [Enterobacter cloacae]
MERGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGKFARTAGFTVSLEGAGTKTMGVIRCDQPRTI
DMAARNGMRLERIPDAVVNEVLARLDAILS

Nucleotide

• Length: 333 bp

>T118348 NZ_CP035738:1740288-1740620 [Enterobacter cloacae]
ATGGAAAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTACTCAT
CGTATCAAAGGCATCATTTAACAAGCTGACCCGGCTACCGGTAGTGGTTCCCGTTACCAGCGGCGGAAAATTTGCTCGTA
CAGCAGGCTTCACCGTTTCACTGGAAGGGGCGGGGACAAAAACAATGGGCGTTATTCGCTGCGATCAGCCCAGAACCATT
GATATGGCGGCCCGGAACGGCATGCGGCTGGAACGTATACCTGATGCCGTTGTAAATGAGGTGCTTGCCAGGCTGGATGC
GATTCTGAGTTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9412.85 Da
• Isoelectric Point: 4.4607

>AT118348 WP_129995436.1 NZ_CP035738:1740030-1740287 [Enterobacter cloacae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTINNGCLIIEPQKRPHYSLEELLAQCDSHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT118348 NZ_CP035738:1740030-1740287 [Enterobacter cloacae]
ATGTATACAACTCGCCTGAAAAAGGTTGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTCGGCATGACCATTAATAATGGCTGCCTGATTATTGAACCCCAAAAGCGGCCTCATTATTCGC
TTGAGGAGCTACTGGCGCAGTGCGACTCGCACGCTGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATCCTGTAA