118345

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-RHH
Location 73141..73757 Replicon chromosome
Accession NZ_CP035738
Organism Enterobacter cloacae strain CZ-1

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag EWI30_RS00365 Protein ID WP_129994705.1
Coordinates 73386..73757 (+) Length 124 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID -
Locus tag EWI30_RS00360 Protein ID WP_129994704.1
Coordinates 73141..73383 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EWI30_RS00335 69119..69718 + 600 WP_129994703.1 glucose-1-phosphatase -
EWI30_RS00340 69712..70593 + 882 WP_114980041.1 virulence factor BrkB family protein -
EWI30_RS00345 70590..71027 + 438 WP_047345532.1 D-tyrosyl-tRNA(Tyr) deacylase -
EWI30_RS00350 71072..72013 + 942 WP_028018144.1 fatty acid biosynthesis protein FabY -
EWI30_RS00355 72025..72942 - 918 WP_114980040.1 alpha/beta hydrolase -
EWI30_RS00360 73141..73383 + 243 WP_129994704.1 ribbon-helix-helix protein, CopG family Antitoxin
EWI30_RS00365 73386..73757 + 372 WP_129994705.1 type II toxin-antitoxin system VapC family toxin Toxin
EWI30_RS00370 73797..74726 - 930 WP_114980037.1 formate dehydrogenase accessory protein FdhE -
EWI30_RS00375 74723..75358 - 636 WP_114980036.1 formate dehydrogenase cytochrome b556 subunit -
EWI30_RS00380 75355..76257 - 903 WP_028018139.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-109)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-49)


Sequences

Toxin        


Download         Length: 124 a.a.        Molecular weight: 13726.83 Da        Isoelectric Point: 6.9790

>T118345 WP_129994705.1 NZ_CP035738:73386-73757 [Enterobacter cloacae]
MEHMAVFDTNILIDLFNNRVEAANTIENTTSHRSISLITWMEVMVGARKHGHEAKTATVLGAFEIIDVTREIAERSVVLR
EKHGMKLPDAIILATAQSRNCPLITRNTKDFQGIPGVVSPYQL

Download         Length: 372 bp

>T118345 NZ_CP035738:73386-73757 [Enterobacter cloacae]
ATGGAACACATGGCGGTTTTTGATACCAATATCCTGATCGACCTTTTCAACAATCGCGTCGAAGCAGCAAATACAATCGA
AAACACAACATCGCACCGGTCAATCAGTCTAATTACATGGATGGAAGTCATGGTGGGGGCACGCAAACATGGCCACGAAG
CGAAAACAGCGACAGTGTTGGGCGCATTTGAGATTATCGATGTAACCCGAGAGATTGCTGAAAGAAGCGTCGTGCTCCGT
GAGAAACATGGCATGAAACTGCCGGATGCCATTATTTTAGCGACTGCCCAAAGCAGAAATTGCCCGTTAATCACCCGGAA
TACCAAAGACTTTCAGGGCATTCCGGGGGTTGTTTCACCGTACCAGCTGTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9451.66 Da        Isoelectric Point: 4.2584

>AT118345 WP_129994704.1 NZ_CP035738:73141-73383 [Enterobacter cloacae]
MNLLAELNMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIEQFLDQQSSSVIRDALGLWGNQQEDGLEYERKLREEW

Download         Length: 243 bp

>AT118345 NZ_CP035738:73141-73383 [Enterobacter cloacae]
ATGAATTTACTCGCGGAGCTTAACATGGGCAGAATTCTGATCGATTTGTCGGATGACGTTATTCAACGTCTCGATAACCT
CAAACAGTTGCGTAACCAACCTCGTGCCGAATTACTGCGTGAAGCAATTGAGCAGTTTCTCGATCAGCAAAGCTCATCCG
TTATCCGTGATGCGCTTGGTCTGTGGGGGAACCAGCAAGAAGACGGATTAGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References