Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/AbrB(antitoxin)
Location 947681..948342 Replicon chromosome
Accession NZ_CP035734
Organism Mycolicibacterium monacense DSM 44395

Toxin (Protein)


Gene name vapC Uniprot ID A0A1X0H4V2
Locus tag EWR22_RS04455 Protein ID WP_011854664.1
Coordinates 947917..948342 (+) Length 142 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1X0H4R4
Locus tag EWR22_RS04450 Protein ID WP_011854663.1
Coordinates 947681..947920 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EWR22_RS04425 (EWR22_04415) 943030..943983 + 954 WP_011854658.1 MlaD family protein -
EWR22_RS04430 (EWR22_04420) 944028..944690 + 663 WP_011854659.1 hypothetical protein -
EWR22_RS04435 (EWR22_04425) 944800..945444 + 645 WP_049771749.1 hypothetical protein -
EWR22_RS04440 (EWR22_04430) 945755..946252 + 498 WP_133056645.1 hypothetical protein -
EWR22_RS04445 (EWR22_04435) 946369..946884 + 516 WP_011854661.1 hypothetical protein -
EWR22_RS29050 (EWR22_04440) 947002..947403 + 402 WP_110807666.1 hypothetical protein -
EWR22_RS04450 (EWR22_04445) 947681..947920 + 240 WP_011854663.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
EWR22_RS04455 (EWR22_04450) 947917..948342 + 426 WP_011854664.1 type II toxin-antitoxin system VapC family toxin Toxin
EWR22_RS04460 (EWR22_04455) 948591..950240 + 1650 WP_011854665.1 NERD domain-containing protein/DEAD/DEAH box helicase -
EWR22_RS29055 951245..951775 - 531 WP_235679620.1 ankyrin repeat domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 920829..948342 27513


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(9-127)

Antitoxin

SpoVT-AbrB domain

(9-43)


Sequences

Toxin        


Download         Length: 142 a.a.        Molecular weight: 14905.99 Da        Isoelectric Point: 9.6917

>T118342 WP_011854664.1 NZ_CP035734:947917-948342 [Mycolicibacterium monacense DSM 44395]
MTPVPLTAVDTSVAVPLLVTSHRQHTMVAKWAKARPLGLSGHALAETYSVLTRLPGDARVTAADAVALIDENFPESFQLG
ARAARAAHREFARRGIAGGATYDGLVALAARERGAVLFTRDARARSTYEALGVSTEVLAEP

Download         Length: 426 bp

>T118342 NZ_CP035734:947917-948342 [Mycolicibacterium monacense DSM 44395]
TTGACGCCGGTGCCACTCACGGCGGTCGATACCAGCGTCGCGGTGCCTCTCCTCGTGACCTCGCACCGGCAACACACCAT
GGTCGCGAAGTGGGCGAAGGCGCGGCCGCTTGGGCTCAGCGGACACGCGCTGGCCGAAACATACTCAGTACTCACGCGCC
TGCCTGGCGACGCTCGCGTCACCGCTGCTGACGCGGTCGCATTGATCGACGAGAACTTTCCCGAATCCTTCCAGCTCGGC
GCGCGGGCCGCGCGCGCCGCCCACCGCGAGTTCGCCCGACGGGGAATCGCCGGGGGTGCAACCTATGACGGGCTGGTTGC
CTTGGCGGCTCGTGAGCGCGGTGCCGTGCTGTTCACCCGAGATGCGCGCGCGAGATCGACCTACGAAGCACTCGGGGTGA
GTACCGAAGTGTTGGCCGAACCATGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 8406.60 Da        Isoelectric Point: 5.7974

>AT118342 WP_011854663.1 NZ_CP035734:947681-947920 [Mycolicibacterium monacense DSM 44395]
MEAVIDSGGRIVLPKQLRDALGLTPGSKVDISAYGGGLHVVPGGRTARLERDEDGRLVARADTVVTDEMMFALIDSGRR

Download         Length: 240 bp

>AT118342 NZ_CP035734:947681-947920 [Mycolicibacterium monacense DSM 44395]
ATGGAGGCTGTTATCGATTCCGGCGGTCGAATCGTCCTGCCCAAGCAGCTGCGCGATGCGCTGGGTCTGACTCCCGGGTC
CAAGGTCGATATTTCTGCGTACGGCGGCGGACTGCACGTCGTTCCCGGAGGGCGAACCGCTCGACTCGAGCGGGATGAGG
ACGGGCGCCTGGTCGCAAGAGCGGATACGGTCGTCACCGATGAGATGATGTTCGCCCTCATCGATTCGGGACGGCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1X0H4V2

Antitoxin

Source ID Structure
AlphaFold DB A0A1X0H4R4

References