Detailed information of TA system TA118336

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2229913..2230447
• Replicon: chromosome
• Accession: NZ_CP035730
• Organism: Sulfolobus sp. S-194

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EWF20_RS11740
• Protein ID: WP_168065965.1
• Coordinates: 2230127..2230447 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: EWF20_RS11735
• Protein ID: WP_168065963.1
• Coordinates: 2229913..2230137 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EWF20_RS11715 (EWF20_11620)	2226345..2226773	-	429	WP_168065955.1	Zn-ribbon domain-containing OB-fold protein	-
EWF20_RS11720 (EWF20_11625)	2226770..2227267	-	498	WP_168065957.1	Zn-ribbon domain-containing OB-fold protein	-
EWF20_RS11725 (EWF20_11630)	2227279..2228454	-	1176	WP_168065959.1	thiolase domain-containing protein	-
EWF20_RS11730 (EWF20_11635)	2228454..2229563	-	1110	WP_168065961.1	thiolase domain-containing protein	-
EWF20_RS11735 (EWF20_11640)	2229913..2230137	+	225	WP_168065963.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
EWF20_RS11740 (EWF20_11645)	2230127..2230447	+	321	WP_168065965.1	PIN domain nuclease	Toxin
EWF20_RS11745 (EWF20_11650)	2230651..2231781	-	1131	WP_168066999.1	acyl-CoA dehydrogenase family protein	-
EWF20_RS11750 (EWF20_11655)	2231849..2233714	+	1866	WP_168065967.1	AMP-binding protein	-
EWF20_RS11755 (EWF20_11660)	2233711..2234526	+	816	WP_168065968.1	amidohydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Post-segregation antitoxin CcdA (5-53)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12387.47 Da
• Isoelectric Point: 4.8010

>T118336 WP_168065965.1 NZ_CP035730:2230127-2230447 [Sulfolobus sp. S-194]
MRDEFLLDASALYPILSYIDKIDVTKIYVIPLTFYKVGNAIWKEYYLKNKDPITLCALFQKFMSKLKLLDNPPAEEIMRV
TSEKELTFYDATYVYSAESYGLFLVS

Nucleotide

• Length: 321 bp

>T118336 NZ_CP035730:2230127-2230447 [Sulfolobus sp. S-194]
ATGAGAGATGAGTTTTTATTAGATGCCTCAGCATTATACCCTATACTTAGCTACATAGATAAGATAGACGTTACTAAGAT
TTATGTAATTCCTTTGACCTTTTACAAAGTTGGAAACGCAATATGGAAGGAATATTATCTGAAAAATAAGGATCCAATCA
CATTATGTGCTCTCTTTCAGAAGTTTATGAGTAAACTTAAACTCCTTGACAATCCACCAGCTGAGGAAATAATGAGAGTT
ACATCAGAAAAAGAACTGACTTTTTACGATGCTACGTACGTTTATTCTGCTGAATCCTATGGGCTATTTTTAGTCTCATA
A

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8928.23 Da
• Isoelectric Point: 4.5901

>AT118336 WP_168065963.1 NZ_CP035730:2229913-2230137 [Sulfolobus sp. S-194]
MSTIISVRVRQELKEKAEKLGINIREVVERALEEAIKEKENEEIEETVRKIKELMKDISEDEWIALIREDRYER

Nucleotide

• Length: 225 bp

>AT118336 NZ_CP035730:2229913-2230137 [Sulfolobus sp. S-194]
ATGTCAACCATAATAAGTGTAAGAGTGAGGCAGGAGTTGAAAGAAAAAGCTGAGAAATTGGGAATTAACATTAGGGAAGT
TGTAGAGAGGGCTTTAGAGGAGGCAATAAAAGAGAAAGAAAATGAGGAGATTGAGGAAACCGTTAGAAAAATTAAGGAAT
TAATGAAGGATATTAGTGAGGATGAATGGATTGCCTTAATAAGGGAAGATAGATATGAGAGATGA