Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3174567..3175221 Replicon chromosome
Accession NZ_CP035720
Organism Escherichia coli strain U15A

Toxin (Protein)


Gene name cptA Uniprot ID F4T2L4
Locus tag U15A_RS15755 Protein ID WP_000244765.1
Coordinates 3174567..3174974 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID F4T2L5
Locus tag U15A_RS15760 Protein ID WP_000354050.1
Coordinates 3174955..3175221 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
U15A_RS15735 3170524..3172257 - 1734 WP_000813195.1 single-stranded-DNA-specific exonuclease RecJ -
U15A_RS15740 3172263..3172973 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
U15A_RS15745 3172998..3173894 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
U15A_RS15750 3174006..3174527 + 522 WP_001055874.1 flavodoxin FldB -
U15A_RS15755 3174567..3174974 - 408 WP_000244765.1 protein YgfX Toxin
U15A_RS15760 3174955..3175221 - 267 WP_000354050.1 FAD assembly factor SdhE Antitoxin
U15A_RS15765 3175464..3176444 + 981 WP_000886084.1 tRNA-modifying protein YgfZ -
U15A_RS15770 3176521..3177180 - 660 WP_000250275.1 hemolysin III family protein -
U15A_RS15775 3177344..3177655 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
U15A_RS15780 3177700..3179133 + 1434 WP_001296350.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16043.95 Da        Isoelectric Point: 11.5202

>T118316 WP_000244765.1 NZ_CP035720:c3174974-3174567 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLIPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T118316 NZ_CP035720:c3174974-3174567 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATTCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10535.07 Da        Isoelectric Point: 5.1599

>AT118316 WP_000354050.1 NZ_CP035720:c3175221-3174955 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRTFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT118316 NZ_CP035720:c3175221-3174955 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCACCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A454A7D7

Antitoxin

Source ID Structure
AlphaFold DB A0A061L3F4

References