Detailed information of TA system TA118268

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /GNAT-DUF1778
• Location: 557087..557853
• Replicon: chromosome
• Accession: NZ_CP035690
• Organism: Grimontia hollisae strain 2013V-1029

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: ET950_RS02530
• Protein ID: WP_154124094.1
• Coordinates: 557356..557853 (+)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: ET950_RS02525
• Protein ID: WP_154124093.1
• Coordinates: 557087..557359 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ET950_RS02490	554281..555483	+	1203	WP_115659929.1	trans-2-enoyl-CoA reductase family protein	-
ET950_RS18885	556330..556967	-	638	Protein_479	hypothetical protein	-
ET950_RS02525	557087..557359	+	273	WP_154124093.1	DUF1778 domain-containing protein	Antitoxin
ET950_RS02530	557356..557853	+	498	WP_154124094.1	GNAT family N-acetyltransferase	Toxin
ET950_RS02535	558232..558882	+	651	WP_154124095.1	hypothetical protein	-
ET950_RS02540	558875..559645	+	771	WP_154124096.1	DUF3307 domain-containing protein	-
ET950_RS02545	559907..560818	-	912	WP_154124097.1	DUF2971 domain-containing protein	-
ET950_RS02550	561091..562631	+	1541	Protein_485	IS21 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (87-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-89)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 18523.23 Da
• Isoelectric Point: 8.8085

>T118268 WP_154124094.1 NZ_CP035690:557356-557853 [Grimontia hollisae]
MMNTVLLDKAKHDRNRFNCGIEALNNYLKVMASQQAKKDNTRTFVLEDDNDNSHIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIVRLAVDERYKGKGLGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQSFQDAENKLFITIADV
RASLG

Nucleotide

• Length: 498 bp

>T118268 NZ_CP035690:557356-557853 [Grimontia hollisae]
ATGATGAATACGGTACTTCTAGATAAAGCTAAACACGATAGAAACCGATTCAACTGTGGCATCGAAGCTCTCAATAATTA
CTTGAAAGTAATGGCGAGTCAGCAAGCAAAGAAAGACAACACCAGAACGTTTGTTTTAGAAGATGATAACGACAACTCAC
ACATCATCGGCTTTTACACACTAACAATGACACCCATTGACTTGAAGGCACTGCCCGATAAGTTACAAAAGAAACACCAA
TCATCCACCTCTGGTGGTCTGATTGTCCGTCTTGCAGTCGATGAGCGATACAAAGGAAAAGGCCTTGGCGAGTGGTTGCT
TATTGACGCACTACGAAAGCTTTTAGCTGCTAGTGATAGCGTTGCATTCCCTGTTGTTATCGTTGATGCCAAAGATGGCG
CAAAACATTTCTATGAACGCTATGGCTTTCAGTCATTTCAAGATGCTGAAAACAAATTATTCATCACCATTGCGGATGTT
CGAGCAAGCTTAGGCTAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 9746.29 Da
• Isoelectric Point: 9.8130

>AT118268 WP_154124093.1 NZ_CP035690:557087-557359 [Grimontia hollisae]
MATTLPRITARVDVDTQDLLTKAAAIAGMSSINSFVLSAAIEKAKQVIEREQALKLSQADAMLLMEALDRPAIQNSKLKA
AANRYESKTQ

Nucleotide

• Length: 273 bp

>AT118268 NZ_CP035690:557087-557359 [Grimontia hollisae]
ATGGCTACTACCTTGCCTCGCATCACCGCTAGAGTTGATGTCGATACACAAGATTTACTTACTAAGGCTGCCGCGATCGC
TGGCATGTCTAGTATAAACTCATTTGTTCTAAGCGCTGCAATTGAAAAAGCTAAACAAGTCATCGAACGTGAACAAGCTT
TAAAGCTGAGCCAAGCTGATGCTATGTTGCTGATGGAAGCTTTAGACCGTCCTGCAATACAGAACTCAAAACTAAAAGCT
GCTGCTAATCGATACGAGAGCAAAACTCAATGA