Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc(toxin)
Location 599978..600549 Replicon chromosome
Accession NZ_CP035689
Organism Vibrio metoecus strain 08-2459

Toxin (Protein)


Gene name doc Uniprot ID Q9KMA2
Locus tag EWA65_RS16550 Protein ID WP_000351248.1
Coordinates 599978..600379 (-) Length 134 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID Q8GNG3
Locus tag EWA65_RS16555 Protein ID WP_001080654.1
Coordinates 600379..600549 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EWA65_RS16505 595152..595700 - 549 WP_095459700.1 hypothetical protein -
EWA65_RS16510 595731..595820 - 90 WP_195708064.1 DUF3265 domain-containing protein -
EWA65_RS16515 595860..596087 - 228 WP_154139272.1 hypothetical protein -
EWA65_RS16520 596262..597395 - 1134 WP_055028476.1 hypothetical protein -
EWA65_RS16525 597622..597792 - 171 Protein_641 DUF645 family protein -
EWA65_RS16530 597976..598098 - 123 WP_086467490.1 DUF3265 domain-containing protein -
EWA65_RS16535 598602..599150 - 549 WP_154139277.1 hypothetical protein -
EWA65_RS16540 599291..599836 - 546 WP_055028470.1 GNAT family N-acetyltransferase -
EWA65_RS16545 599865..600008 - 144 WP_075042602.1 DUF3265 domain-containing protein -
EWA65_RS16550 599978..600379 - 402 WP_000351248.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
EWA65_RS16555 600379..600549 - 171 WP_001080654.1 hypothetical protein Antitoxin
EWA65_RS18895 600790..600876 - 87 Protein_648 DUF645 family protein -
EWA65_RS18900 600882..601025 - 144 Protein_649 DUF645 family protein -
EWA65_RS16570 601272..601808 - 537 WP_000833721.1 cysteine hydrolase -
EWA65_RS18905 602007..602063 - 57 WP_080390559.1 DUF645 family protein -
EWA65_RS18910 602091..602186 - 96 WP_080390557.1 DUF645 family protein -
EWA65_RS16595 602483..602986 - 504 WP_154139280.1 DinB family protein -
EWA65_RS16605 603165..603650 - 486 WP_154139282.1 hypothetical protein -
EWA65_RS16610 603795..604535 - 741 WP_141229596.1 hypothetical protein -
EWA65_RS16615 604688..605002 - 315 WP_055028051.1 DUF4144 domain-containing protein -
EWA65_RS16620 605139..605549 - 411 WP_053313027.1 cytidine deaminase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integron - - 453640..607302 153662
- inside Integrative and Conjugative Element - hlyA 504927..845208 340281


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(1-132)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14608.71 Da        Isoelectric Point: 4.0128

>T118267 WP_000351248.1 NZ_CP035689:c600379-599978 [Vibrio metoecus]
MDIICFPFERVIEINAFILKTEPGMKGAVDIPKLQGALGRIDNAIVYEGLDDVFEIAAKYTACIAVSHALPDANKRTGLA
VALEYLSLNDFELTQENDLLADAVRDLVIGIINETDFADILYAQYAKEQNSAL

Download         Length: 402 bp

>T118267 NZ_CP035689:c600379-599978 [Vibrio metoecus]
ATGGATATCATCTGTTTTCCTTTTGAACGAGTGATCGAGATTAATGCTTTCATCCTAAAAACAGAACCAGGAATGAAAGG
AGCCGTTGATATTCCCAAGCTTCAGGGGGCATTAGGTCGAATCGATAATGCCATCGTTTATGAAGGACTGGATGATGTTT
TTGAAATCGCTGCTAAATACACTGCTTGTATAGCGGTTTCACATGCGCTACCTGATGCGAACAAACGCACAGGTTTGGCT
GTGGCACTTGAATATTTATCACTTAACGATTTTGAGCTTACGCAGGAAAACGATTTACTTGCGGATGCAGTTCGAGATCT
TGTTATCGGCATTATCAACGAAACAGATTTTGCCGACATTCTCTACGCACAATACGCAAAAGAACAAAATTCAGCTCTCT
AA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6287.35 Da        Isoelectric Point: 10.7274

>AT118267 WP_001080654.1 NZ_CP035689:c600549-600379 [Vibrio metoecus]
MNRKVEAYGVDAVERPKIKASKKLDLTGDAGRQIVKSETKLALRTHQKTFTKLADM

Download         Length: 171 bp

>AT118267 NZ_CP035689:c600549-600379 [Vibrio metoecus]
ATGAATCGAAAAGTTGAAGCTTACGGCGTTGATGCTGTTGAAAGACCGAAAATTAAGGCTAGTAAAAAGCTTGATTTGAC
TGGTGATGCTGGTCGTCAAATTGTGAAGTCTGAAACTAAACTTGCTTTGCGTACTCATCAGAAGACATTCACTAAATTGG
CTGATATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0Q0PN34

Antitoxin

Source ID Structure
AlphaFold DB A0A1T4SJJ1

References