Detailed information of TA system TA118259

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 460292..460820
• Replicon: chromosome
• Accession: NZ_CP035689
• Organism: Vibrio metoecus strain 08-2459

Toxin (Protein)

• Gene name: relE
• Uniprot ID: H9L4R3
• Locus tag: EWA65_RS15210
• Protein ID: WP_000221352.1
• Coordinates: 460530..460820 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A0Q0JPP0
• Locus tag: EWA65_RS15205
• Protein ID: WP_019829909.1
• Coordinates: 460292..460540 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EWA65_RS15160	455319..455441	-	123	WP_154139384.1	DUF3265 domain-containing protein	-
EWA65_RS18840	455457..455891	-	435	WP_158003323.1	hypothetical protein	-
EWA65_RS15170	456055..456471	-	417	WP_154139226.1	DUF1311 domain-containing protein	-
EWA65_RS15175	456668..456838	-	171	Protein_393	DUF645 family protein	-
EWA65_RS15180	457152..457547	-	396	WP_195708050.1	DUF3465 domain-containing protein	-
EWA65_RS15185	457702..458475	-	774	WP_055045037.1	NERD domain-containing protein	-
EWA65_RS15190	458504..458617	-	114	WP_141229620.1	DUF3265 domain-containing protein	-
EWA65_RS15195	458611..459552	-	942	WP_154139228.1	hypothetical protein	-
EWA65_RS15200	459757..459938	-	182	Protein_398	DUF645 family protein	-
EWA65_RS15205	460292..460540	+	249	WP_019829909.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
EWA65_RS15210	460530..460820	+	291	WP_000221352.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EWA65_RS15215	460969..461523	-	555	WP_055028162.1	sugar O-acetyltransferase	-
EWA65_RS15220	461689..462153	-	465	WP_154139229.1	acetyltransferase	-
EWA65_RS15225	462347..462529	-	183	WP_055028160.1	DUF645 family protein	-
EWA65_RS15230	462823..463440	-	618	WP_000676400.1	hypothetical protein	-
EWA65_RS15235	463598..464065	-	468	WP_154139230.1	hypothetical protein	-
EWA65_RS15240	464406..464642	+	237	WP_095476849.1	RNA-binding protein	-
EWA65_RS15245	464818..465177	-	360	WP_071170844.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	-	453640..607302	153662

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11247.23 Da
• Isoelectric Point: 10.4678

>T118259 WP_000221352.1 NZ_CP035689:460530-460820 [Vibrio metoecus]
MTYKLEFKKSALKEWKKLAVPLQQQFKKKLIERLENPHVPSAKLSGAENIYKIKLRQSGYRLVYQVENDIIVVTVLAVGK
RERSEVYTKALQRLDD

Nucleotide

• Length: 291 bp

>T118259 NZ_CP035689:460530-460820 [Vibrio metoecus]
ATGACCTATAAGCTTGAGTTCAAAAAGAGTGCTTTGAAGGAATGGAAAAAGTTAGCCGTTCCACTCCAACAGCAGTTCAA
GAAGAAACTAATAGAACGCCTTGAAAATCCACATGTTCCATCAGCCAAACTGTCTGGCGCTGAGAACATTTATAAAATAA
AACTTCGGCAGTCTGGTTATCGCTTAGTCTATCAAGTTGAAAATGACATCATCGTAGTTACTGTTTTAGCTGTCGGTAAA
CGAGAACGCAGCGAAGTTTACACTAAAGCGCTGCAACGGCTAGACGACTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8935.25 Da
• Isoelectric Point: 4.1079

>AT118259 WP_019829909.1 NZ_CP035689:460292-460540 [Vibrio metoecus]
VTTRILADVAASITEFKANPMKVATSAFGAPVAVLNRNEPAFYCVPASTYEIMMDKLEDLELLAITKERLSEDSVSVNID
DL

Nucleotide

• Length: 249 bp

>AT118259 NZ_CP035689:460292-460540 [Vibrio metoecus]
GTGACCACTAGAATCCTGGCTGATGTTGCAGCAAGCATTACCGAATTTAAAGCAAACCCAATGAAAGTTGCAACTAGCGC
CTTCGGTGCTCCTGTTGCTGTACTCAACCGAAATGAGCCGGCTTTCTACTGCGTCCCTGCAAGCACCTACGAGATCATGA
TGGATAAGCTTGAAGATCTTGAGCTACTGGCTATTACCAAAGAACGCTTAAGTGAAGACAGCGTATCGGTAAATATCGAT
GACCTATAA

Structures

Toxin

• AlphaFold DB: H9L4R3

Antitoxin

• AlphaFold DB: A0A0Q0JPP0