Detailed information of TA system TA118223

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 2510357..2511133
• Replicon: chromosome
• Accession: NZ_CP035671
• Organism: Staphylococcus aureus strain VB31683

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: -
• Locus tag: EWG56_RS12950
• Protein ID: WP_000031112.1
• Coordinates: 2510981..2511133 (+)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: W8U4V4
• Locus tag: EWG56_RS12945
• Protein ID: WP_001251224.1
• Coordinates: 2510357..2510956 (+)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EWG56_RS12925	2506275..2507732	+	1458	WP_000649908.1	ABC transporter permease subunit	-
EWG56_RS12930	2507725..2508447	+	723	WP_000590809.1	amino acid ABC transporter ATP-binding protein	-
EWG56_RS12935	2508596..2509723	+	1128	WP_000379980.1	tRNA epoxyqueuosine(34) reductase QueG	-
EWG56_RS12940	2509728..2510198	+	471	WP_000181394.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
EWG56_RS12945	2510357..2510956	+	600	WP_001251224.1	glucosamine-6-phosphate isomerase	Antitoxin
EWG56_RS12950	2510981..2511133	+	153	WP_000031112.1	hypothetical protein	Toxin
EWG56_RS12955	2511968..2512363	+	396	WP_000901023.1	hypothetical protein	-
EWG56_RS12960	2512559..2513944	+	1386	WP_000116232.1	class II fumarate hydratase	-
EWG56_RS12965	2514406..2515227	-	822	WP_000669382.1	RluA family pseudouridine synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5966.31 Da
• Isoelectric Point: 3.8962

>T118223 WP_000031112.1 NZ_CP035671:2510981-2511133 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELSKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T118223 NZ_CP035671:2510981-2511133 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACTGATTTTGAAGATTTAAAAGA
ATTAAGTAAAGAAATGGAACAAATTTCCGATCAAAATGATCAAGAAAAAAATTCTGAAGAAGACAGTCAGTAA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22343.47 Da
• Isoelectric Point: 5.1445

>AT118223 WP_001251224.1 NZ_CP035671:2510357-2510956 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT118223 NZ_CP035671:2510357-2510956 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Structures

Antitoxin

• AlphaFold DB: W8U4V4