Detailed information of TA system TA118219

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2284328..2284857
• Replicon: chromosome
• Accession: NZ_CP035671
• Organism: Staphylococcus aureus strain VB31683

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: EWG56_RS11540
• Protein ID: WP_000621175.1
• Coordinates: 2284495..2284857 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: EWG56_RS11535
• Protein ID: WP_000948330.1
• Coordinates: 2284328..2284498 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EWG56_RS11505	2279364..2279924	+	561	WP_001092400.1	K(+)-transporting ATPase subunit C	-
EWG56_RS11510	2280133..2280612	+	480	WP_001287080.1	hypothetical protein	-
EWG56_RS11515	2280605..2282188	+	1584	WP_001294624.1	PH domain-containing protein	-
EWG56_RS11520	2282175..2282666	+	492	WP_001205918.1	PH domain-containing protein	-
EWG56_RS11525	2282670..2283029	+	360	WP_000581193.1	holo-ACP synthase	-
EWG56_RS11530	2283095..2284243	+	1149	WP_001281159.1	alanine racemase	-
EWG56_RS11535	2284328..2284498	+	171	WP_000948330.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
EWG56_RS11540	2284495..2284857	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
EWG56_RS11550	2285206..2286207	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
EWG56_RS11555	2286327..2286653	+	327	WP_001052491.1	anti-sigma factor antagonist	-
EWG56_RS11560	2286655..2287134	+	480	WP_001190825.1	anti-sigma B factor RsbW	-
EWG56_RS11565	2287109..2287879	+	771	WP_001041107.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T118219 WP_000621175.1 NZ_CP035671:2284495-2284857 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T118219 NZ_CP035671:2284495-2284857 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATTATTCAAAATGACACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAATATAAATTGGATAAAGACTCAGTCATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTATTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6237.75 Da
• Isoelectric Point: 3.8305

>AT118219 WP_000948330.1 NZ_CP035671:2284328-2284498 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNESYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT118219 NZ_CP035671:2284328-2284498 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAATCATATTTATCTTCTAATTCAA
CGAATGAATGA