Detailed information of TA system TA118218

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 2264589..2265103
• Replicon: chromosome
• Accession: NZ_CP035671
• Organism: Staphylococcus aureus strain VB31683

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: T1YA99
• Locus tag: EWG56_RS11450
• Protein ID: WP_000581792.1
• Coordinates: 2264894..2265103 (+)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: -
• Locus tag: EWG56_RS11445
• Protein ID: WP_000138928.1
• Coordinates: 2264589..2264882 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EWG56_RS11420	2260187..2260828	+	642	WP_000483153.1	thiamine phosphate synthase	-
EWG56_RS11425	2260914..2261786	+	873	WP_000725802.1	membrane protein insertase YidC	-
EWG56_RS11435	2262264..2262911	-	648	WP_001187617.1	HD domain-containing protein	-
EWG56_RS11440	2262939..2264423	-	1485	WP_070149277.1	cardiolipin synthase	-
EWG56_RS11445	2264589..2264882	+	294	WP_000138928.1	copper-sensing transcriptional repressor CsoR	Antitoxin
EWG56_RS11450	2264894..2265103	+	210	WP_000581792.1	heavy-metal-associated domain-containing protein	Toxin
EWG56_RS11455	2265231..2265368	+	138	WP_000828354.1	hypothetical protein	-
EWG56_RS11460	2265632..2266834	-	1203	WP_001109944.1	rod shape-determining protein RodA	-
EWG56_RS11465	2267152..2268222	+	1071	WP_000159632.1	D-alanine--D-alanine ligase	-
EWG56_RS11470	2268237..2269595	+	1359	WP_000611469.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (14-92)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7843.77 Da
• Isoelectric Point: 4.1008

>T118218 WP_000581792.1 NZ_CP035671:2264894-2265103 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Nucleotide

• Length: 210 bp

>T118218 NZ_CP035671:2264894-2265103 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGACGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11184.82 Da
• Isoelectric Point: 6.2243

>AT118218 WP_000138928.1 NZ_CP035671:2264589-2264882 [Staphylococcus aureus]
MTEQDIAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVATKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT118218 NZ_CP035671:2264589-2264882 [Staphylococcus aureus]
ATGACTGAACAAGATATTGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGACAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• PDB: 6FF1