Detailed information of TA system TA118195

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 715392..716098
• Replicon: chromosome
• Accession: NZ_CP035668
• Organism: Edwardsiella piscicida strain MS-18-199

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: EVK84_RS03295
• Protein ID: WP_116541156.1
• Coordinates: 715392..715760 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: EVK84_RS03300
• Protein ID: WP_116541155.1
• Coordinates: 715757..716098 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EVK84_RS03285	713333..713575	-	243	WP_071819605.1	integrase	-
EVK84_RS03290	713850..715046	-	1197	WP_015683255.1	tyrosine-type recombinase/integrase	-
EVK84_RS03295	715392..715760	-	369	WP_116541156.1	TA system toxin CbtA family protein	Toxin
EVK84_RS03300	715757..716098	-	342	WP_116541155.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EVK84_RS03305	716116..716337	-	222	WP_000691980.1	DUF987 family protein	-
EVK84_RS03315	716346..716828	-	483	WP_116541154.1	RadC family protein	-
EVK84_RS03320	716837..717295	-	459	WP_116541153.1	antirestriction protein	-
EVK84_RS03325	717381..717539	-	159	WP_116541152.1	DUF905 family protein	-
EVK84_RS03330	717737..718804	-	1068	WP_116541269.1	hypothetical protein	-
EVK84_RS03335	718963..719070	-	108	WP_116541151.1	DUF4339 domain-containing protein	-
EVK84_RS03340	719096..719806	-	711	WP_116541150.1	DeoR family transcriptional regulator	-
EVK84_RS03345	720015..720839	-	825	WP_116541149.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	696747..736556	39809

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (4-103)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13584.81 Da
• Isoelectric Point: 7.7465

>T118195 WP_116541156.1 NZ_CP035668:c715760-715392 [Edwardsiella piscicida]
MKNLPATISLAAKPCLSPVAIWQMLLTRLLEQHYGLMLSDTPFSDETVIKEHIDAGITLANAVNFLVEKYELVRIDRNGF
TSQVQAPYLTATDILQARGACGLMSRCSYREVSNIVLSRSRL

Nucleotide

• Length: 369 bp

>T118195 NZ_CP035668:c715760-715392 [Edwardsiella piscicida]
ATGAAAAATCTACCTGCTACAATTTCGCTGGCGGCGAAGCCCTGTCTGTCGCCCGTTGCAATCTGGCAAATGTTACTTAC
TCGTCTGCTTGAGCAGCACTATGGCCTAATGCTAAGCGACACACCTTTTAGCGATGAAACCGTTATAAAGGAGCATATCG
ATGCCGGTATCACTCTGGCTAATGCCGTTAACTTTCTGGTAGAGAAATATGAGTTAGTTCGTATTGATCGCAATGGATTT
ACCTCTCAGGTACAAGCACCATATTTGACTGCGACAGACATTCTTCAAGCCAGGGGAGCGTGCGGGCTGATGAGCCGGTG
TTCTTACAGGGAGGTAAGTAATATTGTTCTTAGCCGATCACGGCTGTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12747.47 Da
• Isoelectric Point: 6.9463

>AT118195 WP_116541155.1 NZ_CP035668:c716098-715757 [Edwardsiella piscicida]
MSNPTWGLQRDITPCLGARLVQEGNRLHYLADRASITGKFSDAESLKLDVVFPHFISQMESMLTTGEMNPRHAHCVTLYH
NGFNCEADTLGSCGYVYIAIYPPQRKQISREQT

Nucleotide

• Length: 342 bp

>AT118195 NZ_CP035668:c716098-715757 [Edwardsiella piscicida]
ATGAGCAACCCCACATGGGGCCTGCAGCGGGATATCACGCCGTGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCGGCT
GCACTATCTGGCTGACCGGGCAAGTATCACCGGTAAGTTTAGTGATGCCGAAAGCCTGAAGTTGGATGTCGTATTTCCAC
ATTTTATCAGTCAGATGGAATCGATGCTGACCACTGGTGAAATGAATCCCCGCCATGCCCACTGTGTCACCCTGTACCAC
AACGGTTTCAACTGCGAAGCCGATACCCTTGGTAGTTGCGGCTACGTATACATCGCCATTTATCCCCCTCAGCGCAAACA
AATTTCACGAGAGCAAACATGA