Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Txe-Axe/Txe-RelB
Location 225346..225865 Replicon plasmid unnamed1
Accession NZ_CP035661
Organism Enterococcus faecium strain UAMSEF_09

Toxin (Protein)


Gene name Txe Uniprot ID Q848U6
Locus tag EWM55_RS16960 Protein ID WP_000588503.1
Coordinates 225346..225603 (-) Length 86 a.a.

Antitoxin (Protein)


Gene name Axe Uniprot ID Q83WJ8
Locus tag EWM55_RS16965 Protein ID WP_000388479.1
Coordinates 225596..225865 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EWM55_RS16930 220384..220674 - 291 WP_000026576.1 hypothetical protein -
EWM55_RS16935 220810..220989 - 180 WP_001021554.1 hypothetical protein -
EWM55_RS16940 220986..221336 - 351 WP_000568378.1 hypothetical protein -
EWM55_RS16945 221329..222654 - 1326 WP_000969590.1 Y-family DNA polymerase -
EWM55_RS16950 223104..223718 + 615 WP_001261742.1 recombinase family protein -
EWM55_RS16955 223883..224887 - 1005 WP_000455809.1 hypothetical protein -
EWM55_RS16960 225346..225603 - 258 WP_000588503.1 Txe/YoeB family addiction module toxin Toxin
EWM55_RS16965 225596..225865 - 270 WP_000388479.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
EWM55_RS16970 225996..226175 - 180 WP_001120991.1 hypothetical protein -
EWM55_RS16980 226556..227122 - 567 Protein_256 hypothetical protein -
EWM55_RS16985 227198..227877 + 680 WP_002287920.1 IS6-like element IS1216 family transposase -
EWM55_RS16990 227933..229378 - 1446 Protein_258 type I restriction-modification system subunit M -
EWM55_RS16995 229631..230821 - 1191 Protein_259 IS256-like element IS16 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid VanHAX / erm(B) - 1..295052 295052


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-85)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-72)


Sequences

Toxin        


Download         Length: 86 a.a.        Molecular weight: 10206.53 Da        Isoelectric Point: 8.4369

>T118192 WP_000588503.1 NZ_CP035661:c225603-225346 [Enterococcus faecium]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY

Download         Length: 258 bp

>T118192 NZ_CP035661:c225603-225346 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10373.51 Da        Isoelectric Point: 4.4346

>AT118192 WP_000388479.1 NZ_CP035661:c225865-225596 [Enterococcus faecium]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD

Download         Length: 270 bp

>AT118192 NZ_CP035661:c225865-225596 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A828ZXQ8

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZWU7

References