Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 173848..174455 Replicon plasmid unnamed1
Accession NZ_CP035661
Organism Enterococcus faecium strain UAMSEF_09

Toxin (Protein)


Gene name mazF Uniprot ID E3USU0
Locus tag EWM55_RS16640 Protein ID WP_002321032.1
Coordinates 173848..174198 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A829F3C0
Locus tag EWM55_RS16645 Protein ID WP_002287514.1
Coordinates 174192..174455 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EWM55_RS16605 169116..169850 + 735 WP_010729798.1 GntR family transcriptional regulator -
EWM55_RS16610 169834..170544 + 711 WP_010729797.1 GntR family transcriptional regulator -
EWM55_RS16615 170598..171277 + 680 WP_010861579.1 IS6-like element IS1216 family transposase -
EWM55_RS16620 171466..172050 - 585 WP_002299575.1 recombinase family protein -
EWM55_RS16625 172261..172464 - 204 WP_002299573.1 hypothetical protein -
EWM55_RS16630 172474..172896 - 423 WP_002288787.1 hypothetical protein -
EWM55_RS16635 173210..173824 + 615 WP_010729795.1 recombinase family protein -
EWM55_RS16640 173848..174198 - 351 WP_002321032.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
EWM55_RS16645 174192..174455 - 264 WP_002287514.1 hypothetical protein Antitoxin
EWM55_RS16650 174796..174975 - 180 WP_002305761.1 hypothetical protein -
EWM55_RS16660 175356..176063 - 708 WP_002353391.1 hypothetical protein -
EWM55_RS16665 176056..176493 - 438 WP_002296237.1 hypothetical protein -
EWM55_RS16670 176508..176759 - 252 WP_002296238.1 hypothetical protein -
EWM55_RS16675 176759..176965 - 207 WP_002296239.1 hypothetical protein -
EWM55_RS16685 177406..177666 - 261 WP_002296240.1 hypothetical protein -
EWM55_RS16690 178032..178517 - 486 WP_002296241.1 single-stranded DNA-binding protein -
EWM55_RS16695 178520..179419 - 900 WP_002322787.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid VanHAX / erm(B) - 1..295052 295052


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-111)

Antitoxin

Antitoxin PemI-like

(4-87)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13255.28 Da        Isoelectric Point: 8.0280

>T118191 WP_002321032.1 NZ_CP035661:c174198-173848 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Download         Length: 351 bp

>T118191 NZ_CP035661:c174198-173848 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10183.60 Da        Isoelectric Point: 4.6865

>AT118191 WP_002287514.1 NZ_CP035661:c174455-174192 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Download         Length: 264 bp

>AT118191 NZ_CP035661:c174455-174192 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F5H8

Antitoxin

Source ID Structure
AlphaFold DB A0A829F3C0

References