Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 544491..545062 Replicon chromosome
Accession NZ_CP035660
Organism Enterococcus faecium strain UAMSEF_09

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag EWM55_RS03425 Protein ID WP_002286801.1
Coordinates 544721..545062 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag EWM55_RS03420 Protein ID WP_002323011.1
Coordinates 544491..544721 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EWM55_RS03370 539627..540955 + 1329 WP_008265691.1 FAD-containing oxidoreductase -
EWM55_RS03375 540977..541603 + 627 WP_002286816.1 cysteine hydrolase -
EWM55_RS03380 541786..542367 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
EWM55_RS03400 543099..543446 + 348 WP_002286808.1 SOS response-associated peptidase family protein -
EWM55_RS03405 543422..543661 + 240 WP_002321938.1 SOS response-associated peptidase family protein -
EWM55_RS03410 543866..544204 - 339 WP_002286804.1 hypothetical protein -
EWM55_RS03420 544491..544721 + 231 WP_002323011.1 hypothetical protein Antitoxin
EWM55_RS03425 544721..545062 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
EWM55_RS03430 545912..546097 + 186 WP_002304207.1 hypothetical protein -
EWM55_RS03435 546602..546796 + 195 WP_002297028.1 hypothetical protein -
EWM55_RS03440 546954..547236 + 283 Protein_525 transposase -
EWM55_RS03450 547480..548310 - 831 WP_002286789.1 manganese catalase family protein -
EWM55_RS03455 548518..549852 + 1335 WP_002286788.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T118190 WP_002286801.1 NZ_CP035660:544721-545062 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T118190 NZ_CP035660:544721-545062 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT118190 WP_002323011.1 NZ_CP035660:544491-544721 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT118190 NZ_CP035660:544491-544721 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References