Detailed information of TA system TA118187

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 180186..180793
• Replicon: plasmid unnamed1
• Accession: NZ_CP035655
• Organism: Enterococcus faecium strain UAMSEF_08

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: E3USU0
• Locus tag: EWM54_RS15825
• Protein ID: WP_002321032.1
• Coordinates: 180186..180536 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A829F3C0
• Locus tag: EWM54_RS15830
• Protein ID: WP_002287514.1
• Coordinates: 180530..180793 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EWM54_RS15790	175454..176188	+	735	WP_010729798.1	GntR family transcriptional regulator	-
EWM54_RS15795	176172..176882	+	711	WP_010729797.1	GntR family transcriptional regulator	-
EWM54_RS15800	176936..177615	+	680	WP_010861579.1	IS6-like element IS1216 family transposase	-
EWM54_RS15805	177804..178388	-	585	WP_002299575.1	recombinase family protein	-
EWM54_RS15810	178599..178802	-	204	WP_002299573.1	hypothetical protein	-
EWM54_RS15815	178812..179234	-	423	WP_002288787.1	hypothetical protein	-
EWM54_RS15820	179548..180162	+	615	WP_010729795.1	recombinase family protein	-
EWM54_RS15825	180186..180536	-	351	WP_002321032.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
EWM54_RS15830	180530..180793	-	264	WP_002287514.1	hypothetical protein	Antitoxin
EWM54_RS15835	181134..181313	-	180	WP_002305761.1	hypothetical protein	-
EWM54_RS15845	181694..182401	-	708	WP_002353391.1	hypothetical protein	-
EWM54_RS15850	182394..182831	-	438	WP_002296237.1	hypothetical protein	-
EWM54_RS15855	182846..183097	-	252	WP_002296238.1	hypothetical protein	-
EWM54_RS15860	183097..183303	-	207	WP_002296239.1	hypothetical protein	-
EWM54_RS15870	183744..184004	-	261	WP_002296240.1	hypothetical protein	-
EWM54_RS15875	184370..184855	-	486	WP_002296241.1	single-stranded DNA-binding protein	-
EWM54_RS15880	184858..185757	-	900	WP_002322787.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..256961	256961

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-111)

Antitoxin

• Antitoxin PemI-like (4-87)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13255.28 Da
• Isoelectric Point: 8.0280

>T118187 WP_002321032.1 NZ_CP035655:c180536-180186 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Nucleotide

• Length: 351 bp

>T118187 NZ_CP035655:c180536-180186 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10183.60 Da
• Isoelectric Point: 4.6865

>AT118187 WP_002287514.1 NZ_CP035655:c180793-180530 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Nucleotide

• Length: 264 bp

>AT118187 NZ_CP035655:c180793-180530 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Structures

Toxin

• AlphaFold DB: A0A829F5H8

Antitoxin

• AlphaFold DB: A0A829F3C0