Detailed information of TA system TA118182

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 180222..180829
• Replicon: plasmid unnamed1
• Accession: NZ_CP035649
• Organism: Enterococcus faecium strain UAMSEF_01

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: E3USU0
• Locus tag: EWM53_RS15805
• Protein ID: WP_002321032.1
• Coordinates: 180222..180572 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A829F3C0
• Locus tag: EWM53_RS15810
• Protein ID: WP_002287514.1
• Coordinates: 180566..180829 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EWM53_RS15770	175489..176223	+	735	WP_010729798.1	GntR family transcriptional regulator	-
EWM53_RS15775	176207..176917	+	711	WP_010729797.1	GntR family transcriptional regulator	-
EWM53_RS15780	176971..177651	+	681	WP_010861579.1	IS6-like element IS1216 family transposase	-
EWM53_RS15785	177840..178424	-	585	WP_002299575.1	recombinase family protein	-
EWM53_RS15790	178635..178838	-	204	WP_002299573.1	hypothetical protein	-
EWM53_RS15795	178848..179270	-	423	WP_002288787.1	hypothetical protein	-
EWM53_RS15800	179584..180198	+	615	WP_010729795.1	recombinase family protein	-
EWM53_RS15805	180222..180572	-	351	WP_002321032.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
EWM53_RS15810	180566..180829	-	264	WP_002287514.1	hypothetical protein	Antitoxin
EWM53_RS15815	181170..181349	-	180	WP_002305761.1	hypothetical protein	-
EWM53_RS15825	181730..182437	-	708	WP_002353391.1	hypothetical protein	-
EWM53_RS15830	182430..182867	-	438	WP_002296237.1	hypothetical protein	-
EWM53_RS15835	182882..183133	-	252	WP_002296238.1	hypothetical protein	-
EWM53_RS15840	183133..183339	-	207	WP_002296239.1	hypothetical protein	-
EWM53_RS15850	183780..184040	-	261	WP_002296240.1	hypothetical protein	-
EWM53_RS15855	184406..184891	-	486	WP_002296241.1	single-stranded DNA-binding protein	-
EWM53_RS15860	184894..185793	-	900	WP_002322787.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..257028	257028

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-111)

Antitoxin

• Antitoxin PemI-like (4-87)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13255.28 Da
• Isoelectric Point: 8.0280

>T118182 WP_002321032.1 NZ_CP035649:c180572-180222 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Nucleotide

• Length: 351 bp

>T118182 NZ_CP035649:c180572-180222 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10183.60 Da
• Isoelectric Point: 4.6865

>AT118182 WP_002287514.1 NZ_CP035649:c180829-180566 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Nucleotide

• Length: 264 bp

>AT118182 NZ_CP035649:c180829-180566 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Structures

Toxin

• AlphaFold DB: A0A829F5H8

Antitoxin

• AlphaFold DB: A0A829F3C0