Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 544498..545069 Replicon chromosome
Accession NZ_CP035648
Organism Enterococcus faecium strain UAMSEF_01

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag EWM53_RS03150 Protein ID WP_002286801.1
Coordinates 544728..545069 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag EWM53_RS03145 Protein ID WP_002323011.1
Coordinates 544498..544728 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EWM53_RS03095 539634..540962 + 1329 WP_008265691.1 FAD-containing oxidoreductase -
EWM53_RS03100 540984..541610 + 627 WP_002286816.1 cysteine hydrolase -
EWM53_RS03105 541793..542374 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
EWM53_RS03125 543106..543453 + 348 WP_002286808.1 SOS response-associated peptidase family protein -
EWM53_RS03130 543429..543668 + 240 WP_002321938.1 SOS response-associated peptidase family protein -
EWM53_RS03135 543873..544211 - 339 WP_002286804.1 hypothetical protein -
EWM53_RS03145 544498..544728 + 231 WP_002323011.1 hypothetical protein Antitoxin
EWM53_RS03150 544728..545069 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
EWM53_RS03155 545919..546104 + 186 WP_002304207.1 hypothetical protein -
EWM53_RS03160 546609..546803 + 195 WP_002297028.1 hypothetical protein -
EWM53_RS03165 546961..547243 + 283 Protein_524 transposase -
EWM53_RS03175 547487..548317 - 831 WP_002286789.1 manganese catalase family protein -
EWM53_RS03180 548525..549859 + 1335 WP_002286788.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T118181 WP_002286801.1 NZ_CP035648:544728-545069 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T118181 NZ_CP035648:544728-545069 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT118181 WP_002323011.1 NZ_CP035648:544498-544728 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT118181 NZ_CP035648:544498-544728 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References