Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Txe-Axe/Txe-RelB
Location 30016..30535 Replicon plasmid unnamed2
Accession NZ_CP035644
Organism Enterococcus faecium strain UAMSEF_01

Toxin (Protein)


Gene name Txe Uniprot ID Q848U6
Locus tag EWM53_RS00180 Protein ID WP_000588503.1
Coordinates 30278..30535 (+) Length 86 a.a.

Antitoxin (Protein)


Gene name Axe Uniprot ID Q83WJ8
Locus tag EWM53_RS00175 Protein ID WP_000388479.1
Coordinates 30016..30285 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EWM53_RS00145 25063..26250 + 1188 WP_002296840.1 IS256-like element IS16 family transposase -
EWM53_RS00150 26503..27948 + 1446 Protein_27 type I restriction-modification system subunit M -
EWM53_RS00160 28759..29325 + 567 Protein_29 hypothetical protein -
EWM53_RS00170 29706..29885 + 180 WP_001120991.1 hypothetical protein -
EWM53_RS00175 30016..30285 + 270 WP_000388479.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
EWM53_RS00180 30278..30535 + 258 WP_000588503.1 Txe/YoeB family addiction module toxin Toxin
EWM53_RS00185 30994..31998 + 1005 WP_000455809.1 hypothetical protein -
EWM53_RS00190 32163..32777 - 615 WP_001261742.1 recombinase family protein -
EWM53_RS00195 33227..34552 + 1326 WP_000969590.1 Y-family DNA polymerase -
EWM53_RS00200 34545..34895 + 351 WP_000568378.1 hypothetical protein -
EWM53_RS00205 34892..35071 + 180 WP_001021554.1 hypothetical protein -
EWM53_RS00210 35207..35497 + 291 WP_000026576.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid VanHAX - 1..37314 37314


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-85)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-72)


Sequences

Toxin        


Download         Length: 86 a.a.        Molecular weight: 10206.53 Da        Isoelectric Point: 8.4369

>T118179 WP_000588503.1 NZ_CP035644:30278-30535 [Enterococcus faecium]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY

Download         Length: 258 bp

>T118179 NZ_CP035644:30278-30535 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10373.51 Da        Isoelectric Point: 4.4346

>AT118179 WP_000388479.1 NZ_CP035644:30016-30285 [Enterococcus faecium]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD

Download         Length: 270 bp

>AT118179 NZ_CP035644:30016-30285 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A828ZXQ8

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZWU7

References