Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1662949..1663478 Replicon chromosome
Accession NZ_CP035643
Organism Staphylococcus epidermidis strain E73

Toxin (Protein)


Gene name mazF Uniprot ID Q5HME7
Locus tag EVU95_RS08025 Protein ID WP_001829891.1
Coordinates 1662949..1663311 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID Q5HME6
Locus tag EVU95_RS08030 Protein ID WP_001829931.1
Coordinates 1663308..1663478 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EVU95_RS08005 1659951..1660721 - 771 WP_002440602.1 RNA polymerase sigma factor SigB -
EVU95_RS08010 1660696..1661175 - 480 WP_001829903.1 anti-sigma B factor RsbW -
EVU95_RS08015 1661177..1661503 - 327 WP_001829952.1 anti-sigma factor antagonist -
EVU95_RS08020 1661603..1662604 - 1002 WP_001829902.1 PP2C family protein-serine/threonine phosphatase -
EVU95_RS08025 1662949..1663311 - 363 WP_001829891.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
EVU95_RS08030 1663308..1663478 - 171 WP_001829931.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
EVU95_RS08035 1663565..1664713 - 1149 WP_002457107.1 alanine racemase -
EVU95_RS08040 1664780..1665133 - 354 WP_002457108.1 holo-ACP synthase -
EVU95_RS08045 1665181..1665690 - 510 WP_002457109.1 PH domain-containing protein -
EVU95_RS08050 1665677..1667182 - 1506 WP_002457110.1 PH domain-containing protein -
EVU95_RS08055 1667175..1667654 - 480 WP_002457111.1 PH domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13497.65 Da        Isoelectric Point: 9.9522

>T118177 WP_001829891.1 NZ_CP035643:c1663311-1662949 [Staphylococcus epidermidis]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTFLSESKMIEVDNALDISLGLNNFDHHKS

Download         Length: 363 bp

>T118177 NZ_CP035643:c1663311-1662949 [Staphylococcus epidermidis]
ATGATTAGAAGAGGAGATGTTTATTTAGCGGATTTATCACCAGTTCAAGGGTCTGAACAAGGGGGAGTAAGACCTGTAGT
TATCATTCAAAATGATACTGGTAATAAATATAGTCCAACTGTAATTGTAGCTGCGATTACTGGTAGGATTAATAAAGCGA
AAATACCAACCCACGTAGAAATTGAAAAGAAAAAGTATAAATTAGACAAAGATTCAGTTATTCTTCTTGAACAAATTAGA
ACACTAGATAAAAAGCGTTTAAAAGAAAAATTAACATTTTTATCAGAGAGTAAAATGATAGAGGTTGATAATGCCTTAGA
TATTAGTTTGGGATTAAATAACTTTGATCATCATAAATCTTAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6289.92 Da        Isoelectric Point: 4.1920

>AT118177 WP_001829931.1 NZ_CP035643:c1663478-1663308 [Staphylococcus epidermidis]
MLSFNQNRNHSLEQSLKEGYAQMADLNLSLATEAFPIECEACDCNESHLISNSKNE

Download         Length: 171 bp

>AT118177 NZ_CP035643:c1663478-1663308 [Staphylococcus epidermidis]
ATGTTATCTTTTAATCAAAATAGAAACCACAGTCTTGAACAATCTTTAAAAGAAGGTTATGCACAAATGGCCGATTTAAA
CCTCTCCCTAGCAACAGAAGCTTTCCCGATAGAGTGTGAAGCTTGTGATTGCAATGAATCACATTTAATATCTAATTCAA
AGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2G7HWR0

Antitoxin

Source ID Structure
AlphaFold DB A0A0N1EF65

References