Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 84650..85176 Replicon plasmid unnamed4
Accession NZ_CP035636
Organism Enterobacter cloacae strain EN3600

Toxin (Protein)


Gene name relE Uniprot ID A0A7R6HXL1
Locus tag EVV94_RS26975 Protein ID WP_015572079.1
Coordinates 84650..84937 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag EVV94_RS26980 Protein ID WP_000534858.1
Coordinates 84937..85176 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EVV94_RS26950 79789..80793 + 1005 WP_000427614.1 IS110-like element IS5075 family transposase -
EVV94_RS26955 81073..81690 - 618 Protein_82 replication initiation protein -
EVV94_RS26960 82271..83059 - 789 WP_015572118.1 DsbA family protein -
EVV94_RS26965 83088..83696 - 609 WP_094935623.1 hypothetical protein -
EVV94_RS26970 84420..84578 - 159 WP_013087178.1 type I toxin-antitoxin system Hok family toxin -
EVV94_RS26975 84650..84937 - 288 WP_015572079.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EVV94_RS26980 84937..85176 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
EVV94_RS26985 85351..86250 - 900 WP_045325182.1 LysR family transcriptional regulator -
EVV94_RS26990 86478..86828 + 351 WP_040171876.1 hypothetical protein -
EVV94_RS26995 86956..88173 + 1218 WP_072206003.1 MFS transporter -
EVV94_RS27000 88190..89236 + 1047 WP_045325187.1 NAD(P)-dependent alcohol dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..103522 103522


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-83)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11209.09 Da        Isoelectric Point: 10.0714

>T118175 WP_015572079.1 NZ_CP035636:c84937-84650 [Enterobacter cloacae]
MAYFLDFDERALKEWRKLGSTVREQLKNKLAEVLESPRIDANKLRGMHDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T118175 NZ_CP035636:c84937-84650 [Enterobacter cloacae]
ATGGCATATTTTCTGGATTTTGACGAGCGTGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGCTAAA
AAATAAGTTGGCTGAAGTGCTTGAGTCACCCCGAATTGATGCGAACAAGCTTCGGGGTATGCATGACTGTTACAAAATTA
AGCTCCGGTCTTCAGGCTATCGTCTTGTATATCAGGTCATAGACGAGAAGGTTGTCGTCTTTGTGATTTCTGTCGGGAAA
AGAGAGCGCTCGGAAGTTTATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT118175 WP_000534858.1 NZ_CP035636:c85176-84937 [Enterobacter cloacae]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT118175 NZ_CP035636:c85176-84937 [Enterobacter cloacae]
ATGGGTAGCATCAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCTGCGCTTGAAAAAATGGGTGTAACTCC
CTCTGAGGCACTTCGCCTCATGCTTGAATATATTGCTGACAATGAACGTTTGCCGTTCAAACAGACACTCCTGAGTGATG
AGGATGCTGAGCTCGTGGAGATCGTGAAAGAACGACTTCGCAATCCTAAGCCAGTACGTGTGACGCTGGATGAGCTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References