Detailed information of TA system TA118174

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/ElaA-DUF1778
• Location: 38251..38990
• Replicon: plasmid unnamed4
• Accession: NZ_CP035636
• Organism: Enterobacter cloacae strain EN3600

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A411GR51
• Locus tag: EVV94_RS26730
• Protein ID: WP_032664832.1
• Coordinates: 38505..38990 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A411GR70
• Locus tag: EVV94_RS26725
• Protein ID: WP_032664831.1
• Coordinates: 38251..38517 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EVV94_RS26695	33809..34132	+	324	WP_032664828.1	hypothetical protein	-
EVV94_RS26700	34110..34913	-	804	WP_032664829.1	DUF4225 domain-containing protein	-
EVV94_RS26705	35806..36711	-	906	Protein_38	replication initiation protein	-
EVV94_RS26710	36773..37470	+	698	WP_103178130.1	IS1 family transposase	-
EVV94_RS26725	38251..38517	+	267	WP_032664831.1	DUF1778 domain-containing protein	Antitoxin
EVV94_RS26730	38505..38990	+	486	WP_032664832.1	GNAT family N-acetyltransferase	Toxin
EVV94_RS26735	39183..39638	-	456	WP_001166628.1	Hg(II)-responsive transcriptional regulator	-
EVV94_RS26740	39710..40075	+	366	WP_001294656.1	mercuric ion transporter MerT	-
EVV94_RS26745	40091..40366	+	276	WP_000732275.1	mercury resistance system periplasmic binding protein MerP	-
EVV94_RS26750	40394..40819	+	426	WP_000522996.1	organomercurial transporter MerC	-
EVV94_RS26755	40858..42543	+	1686	WP_000209296.1	mercury(II) reductase	-
EVV94_RS26760	42561..42626	+	66	Protein_47	transcriptional regulator MerD	-
EVV94_RS26770	42679..43383	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
EVV94_RS26775	43447..43542	-	96	Protein_49	(4Fe-4S)-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..103522	103522
	-	flank	IS/Tn	-	-	37042..37470	428
	-	flank	IS/Tn	-	-	42679..43383	704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-147)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17593.31 Da
• Isoelectric Point: 9.6084

>T118174 WP_032664832.1 NZ_CP035636:38505-38990 [Enterobacter cloacae]
VGRVTAPEPLSSCHQLAEFASGETVLDDWLKQRGLKNQALGAARTFVVCKANTKQVAGFYSLATGSVNHVEATGSLRRNM
PDPIPVIILARLAVDVSFRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKSFYLHHGFKPSQTQERTLFLKLP
Q

Nucleotide

• Length: 486 bp

>T118174 NZ_CP035636:38505-38990 [Enterobacter cloacae]
GTGGGACGTGTAACAGCACCAGAACCATTATCCAGTTGCCATCAACTTGCTGAGTTCGCCAGTGGTGAGACTGTACTTGA
TGACTGGCTTAAACAGAGAGGATTGAAAAACCAGGCTCTGGGGGCTGCCCGAACGTTCGTAGTTTGTAAAGCAAACACGA
AACAGGTTGCCGGTTTTTACTCTCTGGCCACCGGCAGCGTTAACCACGTGGAAGCAACGGGAAGTCTCAGGCGCAACATG
CCAGATCCTATTCCGGTAATCATACTTGCTCGCCTGGCAGTCGATGTTTCATTCCGGGGAAAGGGGCTCGGGGCCGATTT
ACTGCATGATGCAGTTCTGCGCTGTTATCGTGTAGCTGAGAATATTGGAGTCCGTGCAATTATGGTTCATGCACTGACTG
AAGAAGCCAAAAGTTTTTATCTTCATCATGGCTTTAAGCCATCGCAAACCCAGGAACGTACACTGTTTCTCAAGTTACCA
CAGTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10077.50 Da
• Isoelectric Point: 4.4733

>AT118174 WP_032664831.1 NZ_CP035636:38251-38517 [Enterobacter cloacae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADNVAIDKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT118174 NZ_CP035636:38251-38517 [Enterobacter cloacae]
ATGAAATCAGATGTTCAGCTCAACCTCAGAGCCAAAGAATCTCAAAGAGCACTTATTGATGCTGCAGCAGAGATTCTCCA
TAAATCACGCACAGACTTCATTCTGGAAATGGCATGCCAGGCGGCCGAGAATGTTATCCTCGACCGTCGCGTTTTTAATT
TTAACGATGAGCAGTACGCAGAGTTTATTGATATGCTCGATGCACCAGTTGCGGATAACGTTGCCATTGATAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• AlphaFold DB: A0A411GR51

Antitoxin

• AlphaFold DB: A0A411GR70