Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 141262..142885 Replicon plasmid unnamed2
Accession NZ_CP035634
Organism Enterobacter cloacae strain EN3600

Toxin (Protein)


Gene name hipA Uniprot ID A0A7G9A813
Locus tag EVV94_RS25020 Protein ID WP_020833823.1
Coordinates 141560..142885 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A411GQR5
Locus tag EVV94_RS25015 Protein ID WP_024143029.1
Coordinates 141262..141555 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EVV94_RS24990 136744..137136 + 393 WP_000341066.1 hypothetical protein -
EVV94_RS25000 138092..138739 + 648 WP_008786873.1 hypothetical protein -
EVV94_RS25005 138941..139438 + 498 WP_008786874.1 membrane protein -
EVV94_RS25010 139443..140831 + 1389 WP_008786875.1 hypothetical protein -
EVV94_RS25015 141262..141555 + 294 WP_024143029.1 helix-turn-helix transcriptional regulator Antitoxin
EVV94_RS25020 141560..142885 + 1326 WP_020833823.1 type II toxin-antitoxin system HipA family toxin Toxin
EVV94_RS25025 142946..143152 + 207 WP_000134171.1 hypothetical protein -
EVV94_RS25030 143254..143664 + 411 WP_000985911.1 hypothetical protein -
EVV94_RS25035 143677..144492 + 816 WP_024143028.1 HNH endonuclease -
EVV94_RS25040 144744..145169 + 426 WP_020833821.1 hypothetical protein -
EVV94_RS25045 145717..146025 + 309 WP_001224686.1 hypothetical protein -
EVV94_RS25050 146041..146898 + 858 WP_020833820.1 HNH endonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - vipA/tssB / vipB/tssC 1..289962 289962


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(155-404)

Antitoxin

Cro/C1-type helix-turn-helix domain

(20-70)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50230.15 Da        Isoelectric Point: 6.1424

>T118173 WP_020833823.1 NZ_CP035634:141560-142885 [Enterobacter cloacae]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPEWSADEQGRPLSLSIPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAEHDKAQFFMAQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T118173 NZ_CP035634:141560-142885 [Enterobacter cloacae]
ATGAGCCGTAAACAGCAGCGTCTGGCAATCTGGATGAACGGAATCAAAGTCGGATACTGGGAAAAAAGTAAAGGGGTTGA
CAGCTTACAATACCTTCCTGAATGGAGTGCTGACGAACAGGGAAGGCCGCTGTCATTGTCCATTCCTTTTACTCCCGGGA
ACCAGGTCTGGCGTGGAAATGTGGTACGTGACTATTTTGATAATTTACTCCCGGACAGCGAAGGCATACGCAGACGTCTG
GCAATGCGTTACAAGGCCGATAGCCTGGAGCCTTTTGATCTGCTGACGGAGCTGGGAAAAGACTGCGTGGGCGCGATACA
GCTGCTTCATGACGGAGACGAACCCACAGATTTATATTCCGTGAAGTATCACCCTCTTACTGAATCAGAAATTGCGGCAA
CTTTGCGTAATACCACGGAGACATTGCTACCGGGCAGGCCAGAAGATAACGACGACTTACGTTTATCGATTGCCGGTGCT
CAGGAGAAAACAGCCCTACTGTGGCATGAAGATCGGTGGTGTATGCCGGAGGGTAATACACCAACGACGCATATATTCAA
GCTACCGCTCGGACTTGTGGGGAACATGAAAGCCGACATGAGCTCGTCGGTTGAAAATGAATGGCTCTGCTCGGTACTTC
TCGAACAGTACGGGCTCCCCGTGGCAAGAACGCAGATTGCTCACTTTGAAGATCAGAAAGCGCTGGTGGTAGAACGTTTT
GACCGAAAATGGTCAGGAGACGGACAATGGATCATTCGTTTACCCCAGGAAGATATGTGTCAGGCCCTGGGCGTTTCGCC
ATTACGAAAATATCAGGCGGACGGCGGGCCAGGTATATCGGAAATTATGGAAGTTCTGAGTAATTCAGACCGCGCTGAGC
ATGACAAAGCGCAGTTTTTTATGGCGCAGATAATTTTCTGGATGATGGCCGCAACAGATGGCCACGCTAAAAATTTCAGT
ATTTCTATTGGGCCACAGGGACGATATCACCTAACACCACTCTATGATGTGTTGTCGGCATGGCCGGTGATCGGTCATGG
GAACAATCAGATATCCTGGCAGAAGTGCAAACTGGCGATGGCCGTACGTGGCAGTAGCAACTATTACCAGATATATCGAA
TACAACGACGCCACTGGATCAGACATGGTGAGATAACCGGCCTGAGTAAACAGCAGACAGAGGCGATGATAGAGGAAATC
ATCGCCAGAACCCCAGGTGTCATTGAACGTGTTAGCGGGCTTCTTCCGGACCAGTTCCCCCAGCAGCTTGCGGAAAGTAT
TTTTGATGGAATGAGGCAGCAATGCAGGCGCCTGGCTGAGAAATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10846.21 Da        Isoelectric Point: 10.3134

>AT118173 WP_024143029.1 NZ_CP035634:141262-141555 [Enterobacter cloacae]
INNEYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSVLGVKISFSSTATSSDSK
QTEETLKSNSPARQEKW

Download         Length: 294 bp

>AT118173 NZ_CP035634:141262-141555 [Enterobacter cloacae]
ATCAATAATGAATACCCGTTAAACACGCTCAACCAGCTGCGTCCCTTGCTCATTGGTTTTCGCAAGGCGAATGGCCTGAC
GCAAAAAGACCTGTCCGAAAGGTTAGGCGTCACACAACAGACATACTCACGTCTGGAAGCCAACCCTGCCAGTGCGAGCA
TTGAACGGCTATTCAAGGTGTTTTCCGTCCTAGGTGTAAAAATCAGCTTCTCCTCGACGGCCACCTCTTCAGATAGTAAG
CAGACGGAAGAAACGCTTAAATCAAACTCACCTGCACGACAGGAGAAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7G9A813

Antitoxin

Source ID Structure
AlphaFold DB A0A411GQR5

References