Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-RHH
Location 4229956..4230572 Replicon chromosome
Accession NZ_CP035633
Organism Enterobacter cloacae strain EN3600

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag EVV94_RS21350 Protein ID WP_094935277.1
Coordinates 4229956..4230327 (-) Length 124 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID -
Locus tag EVV94_RS21355 Protein ID WP_094935276.1
Coordinates 4230330..4230572 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EVV94_RS21335 4227456..4228358 + 903 WP_010436855.1 formate dehydrogenase subunit beta -
EVV94_RS21340 4228355..4228990 + 636 WP_010436852.1 formate dehydrogenase cytochrome b556 subunit -
EVV94_RS21345 4228987..4229916 + 930 WP_023309751.1 formate dehydrogenase accessory protein FdhE -
EVV94_RS21350 4229956..4230327 - 372 WP_094935277.1 type II toxin-antitoxin system VapC family toxin Toxin
EVV94_RS21355 4230330..4230572 - 243 WP_094935276.1 ribbon-helix-helix domain-containing protein Antitoxin
EVV94_RS21360 4230772..4231692 + 921 WP_094935275.1 alpha/beta hydrolase -
EVV94_RS21365 4231701..4232642 - 942 WP_039264191.1 fatty acid biosynthesis protein FabY -
EVV94_RS21370 4232687..4233124 - 438 WP_008501836.1 D-tyrosyl-tRNA(Tyr) deacylase -
EVV94_RS21375 4233121..4234002 - 882 WP_033146965.1 virulence factor BrkB family protein -
EVV94_RS21380 4233996..4234595 - 600 WP_094935274.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-109)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-49)


Sequences

Toxin        


Download         Length: 124 a.a.        Molecular weight: 13680.87 Da        Isoelectric Point: 7.3604

>T118172 WP_094935277.1 NZ_CP035633:c4230327-4229956 [Enterobacter cloacae]
MERMAVFDTNILIDLFNNRVEAADAIDRTASHRAISLITWMEVMVGARKHGHEAKTATVMGVFEIIDITRDIAERSVILR
EKHGMKLPDAIILATAQSRSCPLISRNTKDFSGITGVVSPYQL

Download         Length: 372 bp

>T118172 NZ_CP035633:c4230327-4229956 [Enterobacter cloacae]
ATGGAGCGCATGGCGGTGTTCGATACCAATATACTCATCGATCTTTTTAATAATCGCGTTGAGGCTGCGGATGCAATTGA
TCGCACAGCTTCTCATCGAGCCATTAGTCTGATCACATGGATGGAAGTCATGGTGGGGGCCCGCAAACATGGTCACGAAG
CGAAAACTGCGACAGTGATGGGCGTATTTGAGATTATTGATATCACCCGAGATATCGCTGAAAGAAGTGTAATTTTGCGC
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATTCTGGCCACAGCTCAGAGCAGAAGTTGCCCGTTAATCTCACGCAA
TACGAAAGACTTTTCGGGCATCACCGGGGTCGTTTCACCTTACCAGCTGTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9455.65 Da        Isoelectric Point: 4.2803

>AT118172 WP_094935276.1 NZ_CP035633:c4230572-4230330 [Enterobacter cloacae]
MILSADLDMGRILIDLSDDVIQRLDNLKQLRNRPRAELLREAIEQYLDQQSSSVIRDALGLWGNQQEDGLEYERKLREEW

Download         Length: 243 bp

>AT118172 NZ_CP035633:c4230572-4230330 [Enterobacter cloacae]
ATGATTCTTTCCGCGGATCTTGATATGGGCAGAATTCTGATCGATTTGTCTGATGACGTAATTCAACGACTGGATAATCT
GAAGCAGCTGCGTAACCGGCCTCGTGCCGAATTGCTTCGGGAAGCCATTGAGCAATATCTCGACCAGCAGAGTTCATCGG
TTATCCGCGATGCGCTTGGCTTGTGGGGAAACCAGCAGGAAGATGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References