Detailed information of TA system TA118171

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 3582206..3582885
• Replicon: chromosome
• Accession: NZ_CP035633
• Organism: Enterobacter cloacae strain EN3600

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A411GL63
• Locus tag: EVV94_RS18040
• Protein ID: WP_016243829.1
• Coordinates: 3582206..3582547 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A8B2XJW9
• Locus tag: EVV94_RS18045
• Protein ID: WP_016243830.1
• Coordinates: 3582568..3582885 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EVV94_RS18020	3577483..3579228	+	1746	WP_023333458.1	DNA primase	-
EVV94_RS18025	3579382..3581229	+	1848	WP_023309228.1	RNA polymerase sigma factor RpoD	-
EVV94_RS18030	3581337..3581843	-	507	WP_029741755.1	G/U mismatch-specific DNA glycosylase	-
EVV94_RS18040	3582206..3582547	-	342	WP_016243829.1	TA system toxin CbtA family protein	Toxin
EVV94_RS18045	3582568..3582885	-	318	WP_016243830.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EVV94_RS18050	3582906..3583127	-	222	WP_007777714.1	DUF987 domain-containing protein	-
EVV94_RS18055	3583136..3583612	-	477	WP_016243831.1	RadC family protein	-
EVV94_RS18060	3583628..3584086	-	459	WP_016243832.1	antirestriction protein	-
EVV94_RS18065	3584188..3584427	-	240	WP_016243833.1	DUF905 domain-containing protein	-
EVV94_RS18070	3584504..3584971	-	468	WP_016239439.1	hypothetical protein	-
EVV94_RS18075	3584995..3585438	-	444	WP_016243834.1	hypothetical protein	-
EVV94_RS18080	3585438..3585674	-	237	WP_001115854.1	hypothetical protein	-
EVV94_RS18085	3585711..3586412	-	702	WP_047749107.1	WYL domain-containing protein	-
EVV94_RS18090	3586629..3587450	-	822	WP_008324532.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3577483..3619443	41960

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13020.04 Da
• Isoelectric Point: 9.9961

>T118171 WP_016243829.1 NZ_CP035633:c3582547-3582206 [Enterobacter cloacae]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSEERVIQEHIDAGISLADAVNFLVEKYELVRIDRKGF
NWQEQSPYLRAVDILRARQATGLLRQSRKNSVR

Nucleotide

• Length: 342 bp

>T118171 NZ_CP035633:c3582547-3582206 [Enterobacter cloacae]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGCTACTGAC
ACGCCTGCTGGAACAGCACTATGGTCTCACCCTCAACGACACACCGTTCAGTGAGGAACGTGTTATACAGGAACATATCG
ATGCCGGGATCTCGCTGGCTGATGCCGTGAATTTTCTGGTGGAAAAATACGAACTGGTGCGTATCGACAGGAAGGGATTT
AACTGGCAGGAACAATCCCCTTATCTCCGGGCTGTAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAAAAATTCGGTACGGTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 12041.72 Da
• Isoelectric Point: 7.7518

>AT118171 WP_016243830.1 NZ_CP035633:c3582885-3582568 [Enterobacter cloacae]
MTHITWGLQRNITPRLGARLVQEGNRMHYLADRASITGKFSDTECRKLDETFPHFIRQMESMLTTGEMNPRNAHCVTLFH
NGFTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT118171 NZ_CP035633:c3582885-3582568 [Enterobacter cloacae]
ATGACTCACATCACATGGGGCCTGCAGCGGAATATCACGCCGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGGAACCGGAT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACACCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACTGGTGAAATGAATCCCCGCAATGCCCACTGTGTCACCCTGTTCCAC
AACGGTTTCACCTGTGAAGCCGACACCCTTGGCAGTTGCGGTTACGTGTACATCGCCATTTATCCCACCCAGCGCTAA

Structures

Toxin

• AlphaFold DB: A0A411GL63

Antitoxin

• AlphaFold DB: A0A8B2XJW9