Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 586290..586910 Replicon chromosome
Accession NZ_CP035633
Organism Enterobacter cloacae strain EN3600

Toxin (Protein)


Gene name Hha Uniprot ID A0A1H8SI38
Locus tag EVV94_RS03000 Protein ID WP_008499287.1
Coordinates 586290..586508 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag EVV94_RS03005 Protein ID WP_008499288.1
Coordinates 586536..586910 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EVV94_RS02970 582304..582564 + 261 WP_045356432.1 type B 50S ribosomal protein L31 -
EVV94_RS02975 582567..582707 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
EVV94_RS02980 582704..583414 - 711 WP_087822105.1 GNAT family N-acetyltransferase -
EVV94_RS02985 583516..584976 + 1461 WP_094934746.1 PLP-dependent aminotransferase family protein -
EVV94_RS02990 584948..585415 - 468 WP_045400719.1 membrane protein -
EVV94_RS02995 585533..586084 - 552 WP_047061252.1 maltose O-acetyltransferase -
EVV94_RS03000 586290..586508 - 219 WP_008499287.1 hemolysin expression modulator Hha Toxin
EVV94_RS03005 586536..586910 - 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
EVV94_RS03010 587422..590568 - 3147 WP_029741964.1 multidrug efflux RND transporter permease subunit -
EVV94_RS03015 590591..591784 - 1194 WP_029741963.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8612.00 Da        Isoelectric Point: 8.9008

>T118159 WP_008499287.1 NZ_CP035633:c586508-586290 [Enterobacter cloacae]
MSDKPLTKIDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Download         Length: 219 bp

>T118159 NZ_CP035633:c586508-586290 [Enterobacter cloacae]
ATGTCTGATAAACCACTCACAAAAATCGATTATTTGATGCGCTTACGACGTTGTCAGTCAATTGACACGCTTGAGCGCGT
CATTGAAAAGAATAAATACGAGCTTTCTGATAATGAACTGGCGGTATTTTATTCAGCCGCTGACCATCGTCTTGCTGAAC
TGACAATGAATAAACTGTATGACAAAATCCCTACTTCTGTGTGGAAATTTGTCCGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT118159 WP_008499288.1 NZ_CP035633:c586910-586536 [Enterobacter cloacae]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT118159 NZ_CP035633:c586910-586536 [Enterobacter cloacae]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGTAACCATGGCTGGGTAAACGACCCAACGTCTGCCATCAATTTACAGTTGAATGAGCTGATAGAGC
ATATCGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTCATTGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGTTACGGCATTAACGCTCAGGATTTGCAAAAATGGCGTAAATCGGGAAATCGGCT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCGGTGAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1H8SI38

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References