Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 85468..86044 Replicon chromosome
Accession NZ_CP035633
Organism Enterobacter cloacae strain EN3600

Toxin (Protein)


Gene name shpA Uniprot ID A0A4R0G892
Locus tag EVV94_RS00475 Protein ID WP_010427219.1
Coordinates 85468..85755 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A411GC49
Locus tag EVV94_RS00480 Protein ID WP_033144548.1
Coordinates 85742..86044 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EVV94_RS00450 80568..81263 + 696 WP_094935517.1 winged helix-turn-helix domain-containing protein -
EVV94_RS00455 81414..81884 + 471 WP_094935516.1 MarR family transcriptional regulator -
EVV94_RS00460 81881..82948 + 1068 WP_094935515.1 HlyD family secretion protein -
EVV94_RS00465 82953..84029 + 1077 WP_023310256.1 DUF2955 domain-containing protein -
EVV94_RS00470 84026..85297 - 1272 WP_094935514.1 DUF445 domain-containing protein -
EVV94_RS00475 85468..85755 + 288 WP_010427219.1 BrnT family toxin Toxin
EVV94_RS00480 85742..86044 + 303 WP_033144548.1 BrnA antitoxin family protein Antitoxin
EVV94_RS00485 86075..86713 - 639 WP_094935513.1 LysE family translocator -
EVV94_RS00490 86761..87504 - 744 WP_048976422.1 AraC family transcriptional regulator -
EVV94_RS00495 87656..89026 + 1371 WP_094935512.1 NAD-dependent succinate-semialdehyde dehydrogenase -
EVV94_RS00500 89073..89393 - 321 WP_039262264.1 hypothetical protein -
EVV94_RS00505 89393..89938 - 546 WP_023310269.1 porin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(32-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11174.64 Da        Isoelectric Point: 6.7609

>T118158 WP_010427219.1 NZ_CP035633:85468-85755 [Enterobacter cloacae]
MPTEFEWDTNKAKSNLIKHGIRFEEAVLVFDDPYHLSLQDRHENGEFRWQTIGLVHGLIVIMVAHTVRFESGDEVIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

>T118158 NZ_CP035633:85468-85755 [Enterobacter cloacae]
ATGCCAACGGAGTTTGAATGGGATACCAACAAAGCGAAAAGCAATCTCATTAAGCATGGGATCCGCTTTGAAGAAGCTGT
TCTGGTGTTTGACGATCCTTACCATTTATCTTTGCAGGATCGTCATGAAAATGGAGAGTTCCGTTGGCAGACCATTGGCC
TTGTACATGGTCTAATTGTCATTATGGTCGCACACACGGTACGTTTTGAAAGTGGTGACGAAGTCATTCGAATCATTAGC
GCACGAAAGGCGGACCGTAAAGAGAGGAGCCGTTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11395.04 Da        Isoelectric Point: 10.2483

>AT118158 WP_033144548.1 NZ_CP035633:85742-86044 [Enterobacter cloacae]
MSMVKHKRGSAPEMSARREAELKALAEKSEAEIDYSDIPATGDEQWSGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT118158 NZ_CP035633:85742-86044 [Enterobacter cloacae]
ATGAGCATGGTTAAACATAAACGCGGTAGTGCGCCTGAGATGAGTGCTCGCCGTGAGGCTGAGCTCAAAGCACTAGCCGA
AAAATCGGAAGCAGAGATTGATTACAGCGATATCCCTGCCACGGGAGATGAACAGTGGTCAGGGGCGGTGCGAGGGAAGT
TCTATCGGCCACTCAAAACTCAGGCGTCTGTACGTATCGACGCTGACGTGATGGAGTGGCTTAAACGGCCTGGAAAAGGT
TACCAGACGAGGCTCAATGCCATTTTGCGTGAAGCTATGCTGCGCGATCAAAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4R0G892

Antitoxin

Source ID Structure
AlphaFold DB A0A411GC49

References