Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 22958..23492 Replicon chromosome
Accession NZ_CP035633
Organism Enterobacter cloacae strain EN3600

Toxin (Protein)


Gene name relE Uniprot ID A0A6P1V8P2
Locus tag EVV94_RS00165 Protein ID WP_000143805.1
Coordinates 23202..23492 (+) Length 97 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A7I6XZR7
Locus tag EVV94_RS00160 Protein ID WP_000104338.1
Coordinates 22958..23212 (+) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EVV94_RS00140 18313..18762 - 450 WP_045911952.1 hypothetical protein -
EVV94_RS00145 19012..20841 - 1830 WP_025760371.1 hypothetical protein -
EVV94_RS00150 21609..22004 + 396 WP_032672763.1 hypothetical protein -
EVV94_RS00155 22091..22801 + 711 WP_059514180.1 tyrosine-type recombinase/integrase -
EVV94_RS00160 22958..23212 + 255 WP_000104338.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
EVV94_RS00165 23202..23492 + 291 WP_000143805.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EVV94_RS00170 23803..24402 - 600 WP_025760201.1 DUF2913 family protein -
EVV94_RS00175 24422..25144 - 723 WP_025760202.1 NYN domain-containing protein -
EVV94_RS00185 26247..27122 - 876 WP_094935765.1 incFII family plasmid replication initiator RepA -
EVV94_RS00190 27103..27180 - 78 WP_011201829.1 RepA leader peptide Tap -
EVV94_RS00195 27425..27673 - 249 WP_058670686.1 replication regulatory protein RepA -
EVV94_RS00200 27826..28380 - 555 WP_059509465.1 phospholipase D family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-84)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-47)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11463.41 Da        Isoelectric Point: 10.2689

>T118157 WP_000143805.1 NZ_CP035633:23202-23492 [Enterobacter cloacae]
MTFNVEFDERAFKEWNKLEKTIREQFKKKLKKLQQNPYVESARLHGDLAGCFKIKLRASGFRLIYKVIDDEIVIWVVAVG
KREDEKAYETARKRLL

Download         Length: 291 bp

>T118157 NZ_CP035633:23202-23492 [Enterobacter cloacae]
ATGACCTTTAATGTTGAATTTGACGAAAGGGCGTTTAAGGAATGGAATAAGCTCGAAAAGACGATCCGTGAGCAATTCAA
AAAGAAGCTAAAAAAGCTTCAGCAAAACCCTTATGTGGAATCAGCCCGACTGCATGGTGATCTGGCTGGCTGCTTTAAAA
TAAAGCTGAGAGCATCTGGTTTTCGTTTGATTTACAAGGTTATCGATGACGAGATTGTTATATGGGTGGTCGCAGTAGGT
AAACGGGAAGACGAAAAAGCCTATGAAACGGCCCGAAAGCGTCTTTTATAA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9708.04 Da        Isoelectric Point: 4.4247

>AT118157 WP_000104338.1 NZ_CP035633:22958-23212 [Enterobacter cloacae]
MSTIQIRVDDELKKDAYQAFEKLNLSPSDALRLFLRYVAENEKLPFAEVSVMVSEDDEDADILAVVRERLKNPARRIRVN
VDDL

Download         Length: 255 bp

>AT118157 NZ_CP035633:22958-23212 [Enterobacter cloacae]
ATGAGCACAATTCAAATCCGCGTTGATGACGAACTGAAAAAAGACGCCTATCAGGCCTTTGAAAAACTGAATCTGTCGCC
TTCGGACGCACTTCGTCTGTTCCTGCGGTATGTTGCAGAGAACGAAAAACTCCCCTTTGCTGAAGTCTCAGTGATGGTGA
GCGAAGATGATGAAGACGCTGATATTCTGGCAGTTGTTCGGGAAAGACTGAAAAATCCGGCAAGACGGATCAGGGTTAAC
GTTGATGACCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6P1V8P2

Antitoxin

Source ID Structure
AlphaFold DB A0A7I6XZR7

References