Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2685233..2685884 Replicon chromosome
Accession NZ_CP035563
Organism Lacticaseibacillus paracasei strain SRCM103299

Toxin (Protein)


Gene name pemK Uniprot ID K6S8L5
Locus tag EVE90_RS13920 Protein ID WP_003567661.1
Coordinates 2685233..2685616 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID K6RKI5
Locus tag EVE90_RS13925 Protein ID WP_003567665.1
Coordinates 2685636..2685884 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EVE90_RS13915 2684528..2685049 - 522 WP_003591968.1 QueT transporter family protein -
EVE90_RS13920 2685233..2685616 - 384 WP_003567661.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
EVE90_RS13925 2685636..2685884 - 249 WP_003567665.1 antitoxin Antitoxin
EVE90_RS13930 2685952..2687088 - 1137 WP_003591971.1 alanine racemase -
EVE90_RS13935 2687075..2687449 - 375 WP_003567669.1 holo-ACP synthase -
EVE90_RS13940 2687619..2689127 - 1509 WP_119182807.1 DEAD/DEAH box helicase -
EVE90_RS16710 2689253..2689396 - 144 WP_003591975.1 hypothetical protein -
EVE90_RS13945 2689427..2690815 - 1389 WP_003591978.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13773.03 Da        Isoelectric Point: 10.9764

>T118155 WP_003567661.1 NZ_CP035563:c2685616-2685233 [Lacticaseibacillus paracasei]
MDVSVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSTKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

>T118155 NZ_CP035563:c2685616-2685233 [Lacticaseibacillus paracasei]
ATGGATGTGTCCGTAAAACGCGGCGATGTTTTTTTCGCCGATCTGTCTCCGGTTGTCGGCTCCGAACAAGGCGGCAACCG
GCCAGTACTTATTATTCAAAACAACGTTGGCAACCATTACAGTCCCACAGTGATTGTGGCAGCCATTACCTCCAAGATTC
AAAAACCCAAAATGCCGACGCATGTCGGGTTACGGGCCAAGCAAGATGGCGTTGAACGCAACTCGGTCATTTTACTTGAG
CAAATTCGCACGATTGACAAGCAACGGCTACAAGCCCGCGTGACAGCTCTGTCAAGCACCAAAATGGCAGCAGTCGACCG
CGCGTTGGCGATTTCAGTGGGTCTGGTCAGTTTGCCCAAGCCCAAAACATATAATAAGAATTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8938.08 Da        Isoelectric Point: 4.4610

>AT118155 WP_003567665.1 NZ_CP035563:c2685884-2685636 [Lacticaseibacillus paracasei]
MKKQTSVAVTFDDAIKARLDAYCELNDIDAQAVITEAVATFLKSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp

>AT118155 NZ_CP035563:c2685884-2685636 [Lacticaseibacillus paracasei]
ATGAAAAAGCAAACGTCAGTAGCGGTAACGTTTGATGATGCCATTAAGGCGCGGTTGGATGCCTATTGTGAATTGAATGA
CATTGACGCTCAGGCTGTGATTACGGAGGCAGTGGCGACATTTTTGAAGTCACAGGCTCCCGAAATCAACCAATTGATTT
CTGGCTATGTAGCAATGGGCCAGATCAATACAGAGATCAGTCATGCTTTCTCTGCCTGCGAGAGCGAGGCATACGCCCAT
ATTCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K6S8L5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2BNY2

References