Detailed information of TA system TA118151

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 73742..74301
• Replicon: plasmid pO157
• Accession: NZ_CP035546
• Organism: Escherichia coli strain FSIS11705876

Toxin (Protein)

• Gene name: relE
• Uniprot ID: O82928
• Locus tag: EB814_RS27600
• Protein ID: WP_000421248.1
• Coordinates: 74026..74301 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C1J8K5
• Locus tag: EB814_RS27595
• Protein ID: WP_001178089.1
• Coordinates: 73742..74026 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EB814_RS27555	68934..69494	+	561	WP_000840472.1	fertility inhibition protein FinO	-
EB814_RS27560	69627..69839	+	213	WP_001302189.1	ANR family transcriptional regulator	-
EB814_RS27565	70084..70545	+	462	WP_001233856.1	thermonuclease family protein	-
EB814_RS27570	70591..70800	+	210	WP_001302200.1	hemolysin expression modulator Hha	-
EB814_RS27575	70838..71176	+	339	WP_000766796.1	hypothetical protein	-
EB814_RS27580	71416..71670	+	255	WP_000083831.1	replication regulatory protein RepA	-
EB814_RS27585	71906..71980	+	75	WP_001370046.1	RepA leader peptide Tap	-
EB814_RS27590	71973..72830	+	858	WP_000130945.1	incFII family plasmid replication initiator RepA	-
EB814_RS27595	73742..74026	+	285	WP_001178089.1	ribbon-helix-helix domain-containing protein	Antitoxin
EB814_RS27600	74026..74301	+	276	WP_000421248.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EB814_RS27605	74396..74602	+	207	WP_001105064.1	hypothetical protein	-
EB814_RS27610	74702..74917	-	216	Protein_79	transposase	-
EB814_RS27615	74961..75527	-	567	Protein_80	IS3-like element IS600 family transposase	-
EB814_RS27620	75527..75943	-	417	Protein_81	IS91 family transposase	-
EB814_RS27625	75943..76128	-	186	WP_001302179.1	hypothetical protein	-
EB814_RS27630	76305..78515	+	2211	WP_000592771.1	catalase/peroxidase KatP	-
EB814_RS27635	78559..78948	+	390	WP_001172748.1	cytochrome b562 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB / toxB / espP	1..94581	94581

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10735.42 Da
• Isoelectric Point: 9.9324

>T118151 WP_000421248.1 NZ_CP035546:74026-74301 [Escherichia coli]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T118151 NZ_CP035546:74026-74301 [Escherichia coli]
ATGGAACTGAAGTGGAGCAGTAAAGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10474.89 Da
• Isoelectric Point: 5.6558

>AT118151 WP_001178089.1 NZ_CP035546:73742-74026 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT118151 NZ_CP035546:73742-74026 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: A0A2Z6FAZ0

Antitoxin

• AlphaFold DB: A0A731IHA8