Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1895842..1896480 Replicon chromosome
Accession NZ_CP035545
Organism Escherichia coli strain FSIS11705876

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag EB814_RS09325 Protein ID WP_000813794.1
Coordinates 1895842..1896018 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag EB814_RS09330 Protein ID WP_001270286.1
Coordinates 1896064..1896480 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EB814_RS09305 1891463..1892636 - 1174 Protein_1821 BenE family transporter YdcO -
EB814_RS09310 1892728..1893264 + 537 WP_000429145.1 DNA-binding transcriptional regulator SutR -
EB814_RS09315 1893337..1895298 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
EB814_RS09320 1895390..1895620 - 231 WP_000494244.1 YncJ family protein -
EB814_RS09325 1895842..1896018 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
EB814_RS09330 1896064..1896480 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
EB814_RS09335 1896559..1897965 + 1407 WP_000760654.1 PLP-dependent aminotransferase family protein -
EB814_RS09340 1898210..1899355 + 1146 WP_000047432.1 ABC transporter substrate-binding protein -
EB814_RS09345 1899373..1900386 + 1014 WP_000220402.1 ABC transporter ATP-binding protein -
EB814_RS09350 1900387..1901328 + 942 WP_001251315.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T118123 WP_000813794.1 NZ_CP035545:1895842-1896018 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T118123 NZ_CP035545:1895842-1896018 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT118123 WP_001270286.1 NZ_CP035545:1896064-1896480 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT118123 NZ_CP035545:1896064-1896480 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAAGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References