Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 11586..12229 Replicon plasmid pKp199-3
Accession NZ_CP035538
Organism Klebsiella pneumoniae subsp. pneumoniae strain CCRI-22199

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag Kp711_RS01825 Protein ID WP_001044770.1
Coordinates 11813..12229 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag Kp711_RS01820 Protein ID WP_001261282.1
Coordinates 11586..11816 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Kp711_RS01790 7159..8025 - 867 WP_004118283.1 replication initiation protein -
Kp711_RS28385 8560..8664 + 105 WP_032409716.1 hypothetical protein -
Kp711_RS01795 8793..9050 + 258 WP_000764642.1 hypothetical protein -
Kp711_RS01800 9108..9884 - 777 WP_000015958.1 site-specific integrase -
Kp711_RS01805 9881..10624 - 744 WP_000129823.1 hypothetical protein -
Kp711_RS01810 10675..11025 - 351 WP_000493378.1 hypothetical protein -
Kp711_RS01815 11169..11629 - 461 Protein_12 hypothetical protein -
Kp711_RS01820 11586..11816 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
Kp711_RS01825 11813..12229 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
Kp711_RS01830 12303..13013 + 711 WP_004152334.1 ATP-binding protein -
Kp711_RS01835 13047..13744 + 698 Protein_16 IS1 family transposase -
Kp711_RS01840 13761..13952 - 192 Protein_17 nucleotidyltransferase domain-containing protein -
Kp711_RS01845 14022..14576 - 555 WP_063840280.1 AAC(6')-Ib family aminoglycoside 6'-N-acetyltransferase -
Kp711_RS01850 14810..15367 - 558 WP_001217881.1 recombinase family protein -
Kp711_RS01860 15673..16377 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
Kp711_RS01865 16441..16980 + 540 Protein_21 chemotaxis protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid aac(6')-Ib / blaSHV-182 / qnrB9 / aac(3)-IIa - 1..48698 48698


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T118089 WP_001044770.1 NZ_CP035538:11813-12229 [Klebsiella pneumoniae subsp. pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T118089 NZ_CP035538:11813-12229 [Klebsiella pneumoniae subsp. pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT118089 WP_001261282.1 NZ_CP035538:11586-11816 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT118089 NZ_CP035538:11586-11816 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References